The Effects of Negative Pressure Therapy on Hair Growth of Mouse Models.

IF 3.5 3区 医学 Q3 CELL & TISSUE ENGINEERING
Tissue Engineering Part A Pub Date : 2024-11-01 Epub Date: 2024-04-09 DOI:10.1089/ten.TEA.2024.0056
Chun-Yu Cheng, Ming-Huei Cheng, Chin-Yu Yang, Cheng-Han Wang, Joshua Lim, Wei Huang, Chih-Hsin Lin
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Abstract

Negative pressure therapy (NPT) has been shown to facilitate wound healing and promote hair growth in a porcine model. However, there is a paucity of research on the impact of negative pressure on hair growth in murine models. Despite the ability of nude mice to develop hair follicles, the hair they produce is often flawed towing to genetically induced keratin disorders, rendering them a pertinent animal model for assessing hair regeneration. Therefore, this study aims to investigate the effects of negative pressure on hair follicle growth in a nude mouse model. To achieve this, a customized external tissue expansion device was developed to apply negative pressure to the dorsum of nude mice. The mice were subjected to several treatment courses consisting of 15 and 30 min of continuous negative pressure at 10 mmHg, which were repeated 5 and 10 times every other day until sacrifice. Dorsal skin samples were subsequently extracted from the suction and nonsuction areas. The sections were stained with various antibodies to assess the expression of SOX-9, LHX-2, Keratin-15, β-catenin, CD31, and vascular endothelial growth factor-A, and a TUNEL assay was used to analyze cell apoptosis. The results showed that the number of hair follicles and angiogenesis were significantly higher in the suction area than in the nonsuction area in all groups. Moreover, mice that received NPT for 15 min for 10 times had a higher hair follicle density than the other three groups. Immunofluorescence staining for LHX-2 and Keratin 15 further validated the results of these findings. In conclusion, this study demonstrated that negative pressure effectively promotes hair follicle growth and angiogenesis in nude mice through SOX-9- and LHX-2-mediated follicular regeneration and β-catenin-mediated hair follicle morphogenesis. Impact Statement The results of this study indicate that negative pressure therapy (NPT) is effective in promoting hair growth in nude mice, as evidenced by increased hair follicle density and angiogenesis in the treated areas. Using a custom external tissue expansion device (ETED) device, 15-min NPT treatment conducted over 10 sessions demonstrated the highest follicle density. This suggest that developing a regimen for NPT may offer to create innovative treatment approaches for hair loss, ultimately benefiting individuals suffering from hair loss disorders.

负压疗法对小鼠模型毛发生长的影响
在猪模型中,负压疗法(NPT)已被证明可促进伤口愈合和毛发生长。然而,有关负压对小鼠模型毛发生长的影响的研究却很少。尽管裸鼠有发育毛囊的能力,但由于基因诱导的角蛋白紊乱,它们长出的毛发往往有缺陷,因此裸鼠是评估毛发再生的一个相关动物模型。因此,本研究旨在研究负压对裸鼠模型毛囊生长的影响。为此,我们开发了一种定制的体外组织扩张装置(ETED),用于在裸鼠背部施加负压。小鼠接受了几个疗程的治疗,包括 15 和 30 分钟 10 mmHg 的持续负压,每隔一天重复五次和十次,直至牺牲。随后从抽吸区和非抽吸区提取背侧皮肤样本。用各种抗体对切片进行染色,以评估 SOX-9、LHX-2、角蛋白-15、β-catenin、CD31 和 VEGF-A 的表达,并用 TUNEL 检测法分析细胞凋亡。结果表明,在所有组别中,抽吸区(SA)的毛囊数量和血管生成都明显高于非抽吸区(NSA)。此外,接受负压治疗 15 分钟 10 次的小鼠的毛囊密度高于其他三组。对 LHX-2 和角蛋白 15 的免疫荧光染色进一步验证了这些研究结果。总之,这项研究表明,负压通过 SOX-9 和 LHX-2 介导的毛囊再生以及 β-catenin 介导的毛囊形态发生,有效促进了裸鼠的毛囊生长和血管生成。.
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Tissue Engineering Part A
Tissue Engineering Part A Chemical Engineering-Bioengineering
CiteScore
9.20
自引率
2.40%
发文量
163
审稿时长
3 months
期刊介绍: Tissue Engineering is the preeminent, biomedical journal advancing the field with cutting-edge research and applications that repair or regenerate portions or whole tissues. This multidisciplinary journal brings together the principles of engineering and life sciences in the creation of artificial tissues and regenerative medicine. Tissue Engineering is divided into three parts, providing a central forum for groundbreaking scientific research and developments of clinical applications from leading experts in the field that will enable the functional replacement of tissues.
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