Agreement and internal quality assurance of the Neubauer hemocytometer and Makler chamber for human sperm concentration determination.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Ane Francyne Costa, Fabiana Botelho de Miranda Onofre, Alexandre Sherlley Casimiro Onofre
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Abstract

Objective: The Neubauer hemocytometer, as well as the Makler chamber, are devices commonly used in andrology laboratories. The present study aimed to verify if both methods yield comparable results, and whether they can be used interchangeably to determine sperm concentration.

Methods: Sperm and latex beads concentration measurements were performed with the Neubauer hemocytometer and the Makler chamber. Fixed and proportional biases were estimated, and the method agreement was determined by assessing sperm concentration results with the Bland and Altman plot. The Coefficient of Variation (CV) and relative bias were calculated as an index of precision and accuracy, respectively, by measuring latex beads target concentrations in both chambers.

Results: The Makler chamber systematically overestimated the Neubauer hemocytometer concentration measurements by a mean of -7.99%, with limits of agreement (LOA) between -41% to 25.61% (p<0.001). The fixed bias was found for concentration values inferior to 40 x 106/ml range (p<0.001), but not higher concentration results (p>0.05). Measurements with the Neubauer hemocytometer showed the greatest consistency in the study with the CV ranging from 3.01% to 6.67%; while the CV with the Makler chamber ranged from 8.46% to 25.64%. The relative bias for the Neubauer hemocytometer determinations varied from 0.12% to 8.40%, while for the Makler chamber varied from 7.6% to an overestimation of 38.0%.

Conclusions: Measurements made with the Makler chamber demonstrated more variability and a higher degree of overestimation. The Makler chamber is a poor substitute to the Neubauer hemocytometer for evaluation of oligozoospermic samples, although both chambers render similar results for highly concentrated samples.

用于测定人类精子浓度的 Neubauer 血细胞计数器和 Makler 室的一致性和内部质量保证。
目的:Neubauer 血细胞计数器和 Makler 染色体室都是男性学实验室常用的设备。本研究旨在验证这两种方法是否能得出相似的结果,以及它们是否能在测定精子浓度时互换使用:方法:使用 Neubauer 血细胞计数器和 Makler 室测量精子和乳胶珠的浓度。估算了固定偏差和比例偏差,并通过布兰德和阿特曼图评估精子浓度结果来确定方法的一致性。通过测量两个箱中乳胶珠的目标浓度,分别计算出变异系数(CV)和相对偏差,作为精确度和准确度的指标:结果:Makler 试验室系统性地高估了 Neubauer 血球计数器的浓度测量值,平均高估率为 -7.99%,一致度(LOA)在 -41% 至 25.61% 之间(P0.05)。在研究中,使用 Neubauer 血细胞计数器进行的测量显示出最大的一致性,CV 在 3.01% 到 6.67% 之间;而使用 Makler 室进行的测量,CV 在 8.46% 到 25.64% 之间。诺伊鲍尔血细胞计数器测定的相对偏差从 0.12% 到 8.40% 不等,而 Makler 室测定的相对偏差从 7.6% 到 38.0% 不等:结论:使用马克勒室进行的测量显示出更大的变异性和更高的高估程度。在评估少精症样本时,马克勒试验箱不能替代新鲍尔血细胞计数器,尽管两种试验箱对高浓度样本的结果相似。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.30
自引率
6.70%
发文量
56
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