Janus Membranes Patch Achieves High-Quality Tendon Repair: Inhibiting Exogenous Healing and Promoting Endogenous Healing

IF 9.6 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2024-03-27 DOI:10.1021/acs.nanolett.4c00818

Xiaojing Xie, Junjie Xu, Danzhi Ding, Jing Lin*, Kang Han, Chaorong Wang, Fujun Wang*, Jinzhong Zhao* and Lu Wang,

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Abstract

The imbalance between endogenous and exogenous healing is the fundamental reason for the poor tendon healing. In this study, a Janus patch was developed to promote endogenous healing and inhibit exogenous healing, leading to improved tendon repair. The upper layer of the patch is a poly(dl-lactide-co-glycolide)/polycaprolactone (PLGA/PCL) nanomembrane (PMCP-NM) modified with poly(2-methylacryloxyethyl phosphocholine) (PMPC), which created a lubricated and antifouling surface, preventing cell invasion and mechanical activation. The lower layer is a PLGA/PCL fiber membrane loaded with fibrin (Fb) (Fb-NM), serving as a temporary chemotactic scaffold to regulate the regenerative microenvironment. In vitro, the Janus patch effectively reduced 92.41% cell adhesion and 79.89% motion friction. In vivo, the patch inhibited tendon adhesion through the TGF-β/Smad signaling pathway and promoted tendon maturation. This Janus patch is expected to provide a practical basis and theoretical guidance for high-quality soft tissue repair.

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Janus 膜片实现了高质量的肌腱修复：抑制外源性愈合，促进内源性愈合。

内源性愈合和外源性愈合之间的失衡是肌腱愈合不良的根本原因。本研究开发了一种 Janus 补丁，以促进内源性愈合并抑制外源性愈合，从而改善肌腱修复。补片的上层是用聚（2-甲基丙烯酰氧乙基磷脂酰胆碱）（PMPC）修饰的聚（dl-内酯-共聚乙二醇）/聚己内酯（PLGA/PCL）纳米膜（PMCP-NM），可形成润滑防污表面，防止细胞侵入和机械活化。下层是负载纤维蛋白（Fb）的 PLGA/PCL 纤维膜（Fb-NM），作为临时趋化支架调节再生微环境。在体外，Janus 贴片有效减少了 92.41% 的细胞粘附和 79.89% 的运动摩擦。在体内，该贴片通过 TGF-β/Smad 信号通路抑制肌腱粘附，促进肌腱成熟。这种 Janus 补丁有望为高质量的软组织修复提供实践基础和理论指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.