Effect of Moderate Static Magnetic Fields on Mice Oocyte Vitrification: Calcium-Related Genes Expression.

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-10-01 Epub Date: 2024-03-25 DOI:10.1089/bio.2022.0200

Sara Soleimani Pargoo, Farzaneh Baniasadi, Vida Sadat Kazemein Jasemi, Samira Hajiaghalou, Mohsen Gharanfoli, Rouhollah Fathi

{"title":"Effect of Moderate Static Magnetic Fields on Mice Oocyte Vitrification: Calcium-Related Genes Expression.","authors":"Sara Soleimani Pargoo, Farzaneh Baniasadi, Vida Sadat Kazemein Jasemi, Samira Hajiaghalou, Mohsen Gharanfoli, Rouhollah Fathi","doi":"10.1089/bio.2022.0200","DOIUrl":null,"url":null,"abstract":"The ability to cryopreserve oocytes without ultrastructural injury has been a concern in the development and use of methods to preserve female reproduction. The stability of the cell membrane must be preserved to reduce the damage caused by ice crystals during vitrification. One approach that has been explored is the use of static magnetic fields (SMFs), which are believed to influence cell membrane stability. In this study, the in vitro effects of SMF that range between 20-80 mT on the vitrification of mice germinal vesicle (GV) oocytes were studied. The viability and mitochondrial (Mt) membrane potential of both vitrified and nonvitrified oocytes were assessed using Trypan blue and JC1 staining. The high in vitro maturation (IVM) rate and high Mt membrane potential in metaphase II (MII) oocytes were taken into account to determine the optimal magnetic field intensity, that is, 20 mT. None of the SMF conditions resulted in intact spindles in MII oocytes. The study also explored the expression of store-operated calcium entry (Stim1, Orai1, and Ip3r) and meiosis resumption (Ccnb, Cdk) genes in GV and MII oocytes of both vitrified and control groups. The results show that the expressions of Orai1 and Ccnb genes in Vit-MII-SMF oocytes were considerably increased. However, no significant difference in Stim1 expression was observed between the groups. The Vit-MII-SMF group exhibited a significantly higher Ccnb expression compared to other groups. In vitro fertilization (IVF) was performed to evaluate the 2 pronuclear (2PN) rates. The findings demonstrated that using 20 mT SMF improved 2PN rates compared to the nonvitrified groups. This study provides a deeper understanding of the effects of moderate SMF and vitrification on the expression of calcium channel genes in GV and MII oocytes. The results suggest that applying a 20 mT SMF can help prevent cryoinjury and enhance the characteristics of vitrified-warmed oocytes.","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"441-451"},"PeriodicalIF":1.6000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biopreservation and Biobanking","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1089/bio.2022.0200","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/25 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

The ability to cryopreserve oocytes without ultrastructural injury has been a concern in the development and use of methods to preserve female reproduction. The stability of the cell membrane must be preserved to reduce the damage caused by ice crystals during vitrification. One approach that has been explored is the use of static magnetic fields (SMFs), which are believed to influence cell membrane stability. In this study, the in vitro effects of SMF that range between 20-80 mT on the vitrification of mice germinal vesicle (GV) oocytes were studied. The viability and mitochondrial (Mt) membrane potential of both vitrified and nonvitrified oocytes were assessed using Trypan blue and JC1 staining. The high in vitro maturation (IVM) rate and high Mt membrane potential in metaphase II (MII) oocytes were taken into account to determine the optimal magnetic field intensity, that is, 20 mT. None of the SMF conditions resulted in intact spindles in MII oocytes. The study also explored the expression of store-operated calcium entry (Stim1, Orai1, and Ip3r) and meiosis resumption (Ccnb, Cdk) genes in GV and MII oocytes of both vitrified and control groups. The results show that the expressions of Orai1 and Ccnb genes in Vit-MII-SMF oocytes were considerably increased. However, no significant difference in Stim1 expression was observed between the groups. The Vit-MII-SMF group exhibited a significantly higher Ccnb expression compared to other groups. In vitro fertilization (IVF) was performed to evaluate the 2 pronuclear (2PN) rates. The findings demonstrated that using 20 mT SMF improved 2PN rates compared to the nonvitrified groups. This study provides a deeper understanding of the effects of moderate SMF and vitrification on the expression of calcium channel genes in GV and MII oocytes. The results suggest that applying a 20 mT SMF can help prevent cryoinjury and enhance the characteristics of vitrified-warmed oocytes.

查看原文本刊更多论文

中度静态磁场对小鼠卵母细胞玻璃化的影响：钙相关基因的表达。

冷冻保存卵母细胞而不造成超微结构损伤的能力，一直是开发和使用女性生殖保存方法时所关注的问题。必须保持细胞膜的稳定性，以减少玻璃化过程中冰晶造成的损伤。静态磁场（SMF）被认为能影响细胞膜的稳定性，是目前探索的一种方法。本研究研究了 20-80 mT 的静态磁场对小鼠生殖囊卵母细胞玻璃化的体外影响。使用胰蓝和 JC1 染色法评估了玻璃化和非玻璃化卵母细胞的存活率和线粒体（Mt）膜电位。在确定最佳磁场强度（即 20 mT）时，考虑到了成熟期 II（MII）卵母细胞的高体外成熟（IVM）率和高 Mt 膜电位。没有一种 SMF 条件能使 MII 卵母细胞中的纺锤体完好无损。研究还探讨了玻璃化组和对照组的 GV 和 MII 卵母细胞中储存操作钙离子进入（Stim1、Orai1 和 Ip3r）和减数分裂恢复（Ccnb、Cdk）基因的表达情况。结果显示，Vit-MII-SMF 卵母细胞中 Orai1 和 Ccnb 基因的表达量显著增加。然而，各组之间的 Stim1 表达量并无明显差异。与其他组相比，Vit-MII-SMF 组的 Ccnb 表达量明显更高。体外受精（IVF）是为了评估2个原核（2PN）的比率。研究结果表明，与非玻璃化组相比，使用 20 mT SMF 可提高 2PN 率。这项研究加深了人们对适度 SMF 和玻璃化对 GV 和 MII 卵母细胞中钙离子通道基因表达的影响的理解。结果表明，使用 20 mT SMF 有助于防止冷冻损伤，并增强玻璃化温育卵母细胞的特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biopreservation and Biobanking Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology

自引率

12.50%

发文量

114

期刊介绍： Biopreservation and Biobanking is the first journal to provide a unifying forum for the peer-reviewed communication of recent advances in the emerging and evolving field of biospecimen procurement, processing, preservation and banking, distribution, and use. The Journal publishes a range of original articles focusing on current challenges and problems in biopreservation, and advances in methods to address these issues related to the processing of macromolecules, cells, and tissues for research. In a new section dedicated to Emerging Markets and Technologies, the Journal highlights the emergence of new markets and technologies that are either adopting or disrupting the biobank framework as they imprint on society. The solutions presented here are anticipated to help drive innovation within the biobank community. Biopreservation and Biobanking also explores the ethical, legal, and societal considerations surrounding biobanking and biorepository operation. Ideas and practical solutions relevant to improved quality, efficiency, and sustainability of repositories, and relating to their management, operation and oversight are discussed as well.