Evaluation of the histopathological effects of injectable hyaluronic acid as a filler material in rat’s lip

IF 0.4 Q4 DENTISTRY, ORAL SURGERY & MEDICINE
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Abstract

Objectives

The present work aimed to examine the early and late histopathological changes occurring in the lower lips of rats, as a result of hyaluronic acid injection, and to determine whether it could really bring us closer to the ideal soft tissue filler material. This research highlights the importance of experimental studies on this subject, since adverse reactions have been observed routinely in dental practice.

Material and methods

The present study was carried out on 36 adult male albino rats and was divided into two equal groups, each consisting of 18 rats; group I (control group) and group II (experimental group). Rats in the experimental group were injected with hyaluronic acid (0.07 ml), whereas rats in the control group were injected with 0.9% sodium chloride.

Results

The results showed inflammation triggered by the injection of hyaluronic acid. On examining the specimens histologically using Hematoxylin and Eosin stains, Toluidine Blue stain and immunohistochemistry with anti-CD68 antibody, there was a significantly greater inflammatory response in the groups at early interval with hyaluronic acid compared with the control (P < 0.0001). Higher collagen formation was also observed with Masson’s Trichrome staining.

Conclusion

The undesirable effects of hyaluronic acid filler injection can be detected at early intervals following its injection. Fortunately, all the undesirable effects are transient and decrease by time, reaching levels comparable to normal. Overall, HA was well-tolerated by tissues, reflecting how this filler material possesses a biocompatible property.

评估注射用透明质酸作为填充材料对大鼠嘴唇的组织病理学影响
本研究旨在探讨透明质酸注射后大鼠下嘴唇早期和晚期的组织病理学变化,并确定透明质酸是否真的能让我们更接近理想的软组织填充材料。本研究以 36 只成年雄性白化大鼠为对象,分为两组,每组 18 只;第一组(对照组)和第二组(实验组)。实验组大鼠注射透明质酸(0.07 毫升),对照组大鼠注射 0.9% 氯化钠。用苏木精和伊红染色法、甲苯胺蓝染色法和抗 CD68 抗体免疫组化法对标本进行组织学检查后发现,与对照组相比,注射玻尿酸组在早期出现的炎症反应明显更大(P <0.0001)。结论透明质酸填充剂注射后的不良反应可在早期发现。幸运的是,所有不良反应都是短暂的,随着时间的推移会逐渐减轻,达到与正常水平相当的程度。总体而言,组织对透明质酸的耐受性良好,这反映出这种填充材料具有生物相容性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
0.80
自引率
0.00%
发文量
129
审稿时长
83 days
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