Identification of Canadian Barley Varieties by High-throughput SNP Genotyping

IF 1 4区农林科学 Q3 AGRONOMY

Canadian Journal of Plant Science Pub Date : 2024-03-12 DOI:10.1139/cjps-2023-0187

Sung-Jong Lee, Maria Eckhardt, Mathieu Dusabenyagasani, Marta Izydorczyk, T. Demeke, Daniel Perry, S. Walkowiak

引用次数: 0

Abstract

Barley is a major grain crop in Canada and is used for generating malt, in addition to being used as human food and animal feed. Differentiating barley varieties is important for malt quality assurance and grain handling. Here, we present a DNA-based testing method for the identification of Canadian barley varieties. The method uses 24 custom TaqMan genotyping assays, which are analyzed using the high-throughput SmartChip system (Takara Bio Inc.). Using this method, we are able to distinguish 124 barley varieties commonly grown in Canada.

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通过高通量 SNP 基因分型鉴定加拿大大麦品种

大麦是加拿大的主要粮食作物，除用作人类食物和动物饲料外，还用于生产麦芽。区分大麦品种对于麦芽质量保证和谷物处理非常重要。在此，我们介绍一种基于 DNA 的加拿大大麦品种鉴定测试方法。该方法使用 24 种定制的 TaqMan 基因分型测定，并使用高通量 SmartChip 系统（Takara Bio 公司）进行分析。利用这种方法，我们能够区分加拿大通常种植的 124 个大麦品种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Canadian Journal of Plant Science 生物-农艺学

CiteScore

1.90

自引率

8.30%

发文量

审稿时长

1 months

期刊介绍： Published since 1957, the Canadian Journal of Plant Science is a bimonthly journal that contains new research on all aspects of plant science relevant to continental climate agriculture, including plant production and management (grain, forage, industrial, and alternative crops), horticulture (fruit, vegetable, ornamental, greenhouse, and alternative crops), and pest management (entomology, plant pathology, and weed science). Cross-disciplinary research in the application of technology, plant breeding, genetics, physiology, biotechnology, microbiology, soil management, economics, meteorology, post-harvest biology, and plant production systems is also published. Research that makes a significant contribution to the advancement of knowledge of crop, horticulture, and weed sciences (e.g., drought or stress resistance), but not directly applicable to the environmental regions of Canadian agriculture, may also be considered. The Journal also publishes reviews, letters to the editor, the abstracts of technical papers presented at the meetings of the sponsoring societies, and occasionally conference proceedings.