Evaluation of serum pro/anti-angiogenic biomarkers in hyperglycemic rats treated with Securigera securidaca seeds, alone and in combination with Glibenclamide

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-13 DOI:10.34172/jcvtr.32960

E. Bahreini, Mohammad Babaei, Forogh Mohammadi, Shahin Alizadeh-Fanalou

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引用次数: 0

Abstract

Introduction: Herbal medicines are commonly used by many people with diabetes in addition to standard treatment. Plants contain numerous known and unknown compounds that may exacerbate or ameliorate diabetes complications. Therefore, it is crucial to be aware of the side effects of these herbs before prescribing them. This study aimed to investigate the effects of hydroalcoholic extracts of Securigera securidaca (HESS) seeds alone and in combination with glibenclamide on the angiogenic/anti-angiogenic balance in streptozotocin (STZ)-induced diabetic rats. Methods: Groups involved in this animal study included diabetic and healthy controls, three doses of HESS, glibenclamide, and combination therapy. Serum samples were collected and analyzed for a vascular endothelial growth factor (VEGF), fibroblast growth factor 21 (FGF21), fetal liver kinase 1 (FLK-1), soluble fms-like tyrosine kinase 1 (sFLT-1), and transforming growth factor -beta (TGF-β). Results: Induction of diabetes increased VEGF, FGF21, and TGF-β serum levels and decreased circulating FLK-1 and sFLT-1 factors. Herbal extract, except TGF-β, had little effect on the above blood levels even at the highest doses. Glibenclamide was more effective than the highest dose of HESS in improving the vascular complications of diabetes. Combination therapy with the highest dose of HESS partly enhanced the glibenclamide effects. Conclusion: Compared with glibenclamide as a standard chemical drug, HESS had no significant effects on the blood levels of the pro/anti-angiogenesis factor in diabetic rats. Glibenclamide attenuated the levels of the biomarkers and its effects were somewhat enhanced in combination with the highest dose of HESS.

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评估单独或与格列本脲联合使用 Securigera securidaca 种子治疗高血糖大鼠的血清促/抗血管生成生物标志物

导言：除标准治疗外，许多糖尿病患者还经常使用草药。植物中含有许多已知和未知的化合物，可能会加重或减轻糖尿病并发症。因此，在处方这些草药之前，了解其副作用至关重要。本研究旨在探讨 Securigera securidaca（HESS）种子的水醇提取物单独使用或与格列本脲联合使用对链脲佐菌素（STZ）诱导的糖尿病大鼠血管生成/抗血管生成平衡的影响。方法：本动物研究涉及的组别包括糖尿病对照组和健康对照组、三种剂量的 HESS、格列本脲和联合疗法。收集血清样本并分析血管内皮生长因子（VEGF）、成纤维细胞生长因子 21（FGF21）、胎肝激酶 1（FLK-1）、可溶性 fms 样酪氨酸激酶 1（sFLT-1）和转化生长因子-β（TGF-β）。结果诱发糖尿病会增加血管内皮生长因子、FGF21 和 TGF-β 的血清水平，降低循环中的 FLK-1 和 sFLT-1 因子。除 TGF-β 外，草药提取物对上述血药浓度的影响甚微，即使使用最高剂量也是如此。在改善糖尿病血管并发症方面，格列本脲比最高剂量的 HESS 更有效。与最高剂量的 HESS 联合治疗可部分增强格列本脲的效果。结论与作为标准化学药物的格列本脲相比，HESS对糖尿病大鼠血液中促/抗血管生成因子的水平没有显著影响。格列本脲可减轻生物标志物的水平，与最高剂量的 HESS 合用时，格列本脲的作用有所增强。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.