Genotypic Detection of Carbapenems Resistance Genes in Acinetobacter baumannii Isolated from Urinary Tract Infection Patients

Bionatura Pub Date : 2024-03-15 DOI:10.21931/rb/2024.09.01.60

T. Hayder Hasan, Ameer Sadeq Yasir AL-Ethari, S. Ghani Al-Muhanna, Israa Abdul Ameer Al-Kraety

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Abstract

Acinetobacter baumannii is a Gram-negative bacterium characterized by its short, round, rod-shaped morphology. It is an opportunistic pathogen that poses a significant threat, particularly to immunocompromised patients, often those with hospital stays lasting less than 90 days. Between June 2022 and July 2023, 214 urine samples were collected from individuals suspected of having urinary tract infections (UTIs). These samples were subjected to antibiotic resistance testing, focusing on detecting specific genes related to carbapenem resistance, namely blaNDM, blaKPC, and blaVIM.The study's results revealed a notable trend in antibiotic resistance among the bacterial isolates. Ceftazidime, cefotaxime, and ceftriaxone, commonly used antibiotics for UTIs, showed a high resistance rate among the tested isolates. This resistance highlights the challenges healthcare professionals face when treating UTIs caused by Acinetobacter baumannii. On the other hand, the isolates displayed a comparatively lower resistance rate to imipenem and meropenem, two necessary carbapenem antibiotics. This lower resistance to carbapenems is encouraging as these drugs are often considered the last line of defense against multidrug-resistant bacterial infections. The presence of carbapenem resistance genes, such as blaNDM, blaKPC, and blaVIM, in the Acinetobacter baumannii isolates is of particular concern. These genes confer resistance to carbapenem antibiotics, crucial for treating severe infections caused by multidrug-resistant bacteria. In conclusion, the study aims to study the growth of antibiotic resistance in Acinetobacter baumannii, especially in urinary tract infections in immunocompromised patients with more extended hospital stays. It also highlights the need for Surveys and periodic examinations to detect the spread of bacteria and their resistance. Keywords: Carbapenems, UTI, genes, blaNDM, blaKPC, and blaVIM.

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从泌尿道感染患者中分离出的鲍曼不动杆菌耐碳苯纳西林基因的基因型检测

鲍曼不动杆菌（Acinetobacter baumannii）是一种革兰氏阴性细菌，其特征是短小、圆形、杆状形态。它是一种机会性病原体，尤其对免疫力低下的患者（通常是住院时间少于 90 天的患者）构成重大威胁。2022 年 6 月至 2023 年 7 月期间，从疑似尿路感染（UTI）患者身上采集了 214 份尿液样本。这些样本接受了抗生素耐药性测试，重点检测与碳青霉烯耐药性有关的特定基因，即 blaNDM、blaKPC 和 blaVIM。头孢他啶、头孢噻肟和头孢曲松是治疗UTI的常用抗生素，但在检测的分离菌株中耐药率很高。这种耐药性凸显了医护人员在治疗鲍曼不动杆菌引起的UTI时所面临的挑战。另一方面，这些分离物对亚胺培南和美罗培南这两种必要的碳青霉烯类抗生素的耐药率相对较低。对碳青霉烯类抗生素的耐药性降低令人鼓舞，因为这类药物通常被认为是抵抗耐多药细菌感染的最后一道防线。鲍曼不动杆菌分离物中出现的碳青霉烯类耐药基因（如 blaNDM、blaKPC 和 blaVIM）尤其令人担忧。这些基因可产生对碳青霉烯类抗生素的耐药性，而碳青霉烯类抗生素是治疗由耐多药细菌引起的严重感染的关键。总之，该研究旨在研究鲍曼不动杆菌耐药性的增长情况，尤其是在住院时间较长的免疫力低下患者的尿路感染中。研究还强调了调查和定期检查的必要性，以检测细菌的传播及其耐药性：碳青霉烯类、UTI、基因、blaNDM、blaKPC 和 blaVIM。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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