Anti-Aging Properties of Cannabis sativa Leaf Extract against UVA Irradiation

IF 3.4 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cosmetics Pub Date : 2024-03-18 DOI:10.3390/cosmetics11020045

Kunlathida Luangpraditkun, Preeyanuch Pimjuk, Preeyawass Phimnuan, Wisanee Wisanwattana, Chothip Wisespongpand, N. Waranuch, J. Viyoch

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Abstract

Hemp extract has garnered interest as a potential cosmeceutical agent with multifunctional activities, particularly in protecting against UV-induced skin cell aberrations and restoring aged skin cells. The ethanolic extract of Cannabis sativa leaves was prepared into an aqueous solution (CLES) to investigate its anti-photoaging ability. HPLC analysis revealed that the CLES contained 1.64 ± 0.01% w/w of cannabidiol and 0.11% w/w of ∆9-tetrahydrocannabinol. Additionally, the total phenolic content was found to be 4.08 ± 0.30 mg gallic acid equivalent per g of solution using the Folin–Ciocalteu method. The CLES exhibited potent scavenging activity using a DPPH assay, with an EC50 value of 277.9 ± 2.41 μg/mL, comparable to L-ascorbic acid, with 2.19 ± 0.28 μg/mL. The anti-photoaging potential of the CLES was evaluated using UVA-irradiated and in vitro-aged fibroblasts as a model. Pre-treatment with 20 μg/mL CLES for 24 h significantly alleviated the reduction in type I procollagen and suppressed the overproduction of MMP-1 and IL-6 induced by UVA. Moreover, the percentage of senescence-associated β-galactosidase-expressing cells decreased significantly to 11.9 ± 0.5% in the aged cells treated with CLES compared with untreated cells (18.8 ± 3.8%). These results strongly indicate the cosmeceutical potential of the CLES as an effective active agent for the anti-photoaging prevention and/or treatment.

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大麻叶提取物抗 UVA 辐射的抗衰老特性

大麻提取物作为一种具有多功能活性的潜在药用化妆品，尤其是在防止紫外线引起的皮肤细胞畸变和修复老化的皮肤细胞方面，已经引起了人们的兴趣。我们将大麻叶乙醇提取物制成水溶液（CLES），以研究其抗光老化能力。HPLC 分析显示，CLES 含有 1.64 ± 0.01% w/w 的大麻二酚和 0.11% w/w 的∆9-四氢大麻酚。此外，用 Folin-Ciocalteu 法检测发现，每克溶液中的总酚含量为 4.08 ± 0.30 毫克没食子酸当量。在 DPPH 试验中，CLES 表现出了强大的清除活性，其 EC50 值为 277.9 ± 2.41 μg/mL，与 L-抗坏血酸（2.19 ± 0.28 μg/mL）相当。以 UVA 照射和体外老化的成纤维细胞为模型，对 CLES 的抗光照老化潜力进行了评估。用 20 μg/mL CLES 预处理 24 小时可显著缓解 I 型胶原蛋白的减少，并抑制 UVA 诱导的 MMP-1 和 IL-6 的过度生成。此外，与未处理的细胞（18.8 ± 3.8%）相比，经 CLES 处理的老化细胞中与衰老相关的 β-半乳糖苷酶表达细胞的百分比明显降低至 11.9 ± 0.5%。这些结果有力地说明了 CLES 作为一种有效的活性剂在预防和/或治疗抗光老化方面的药用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cosmetics Medicine-Surgery

CiteScore

5.20

自引率

12.10%

发文量

108

审稿时长

8 weeks