Variability of salivary analytes under daily conditions and their implications for periodontitis biomarkers

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-18 DOI:10.3389/fdmed.2024.1369186

Amanda Carolina Souza Delfino Rocha, R. Orlandini, Ana Carolina Fragoso Motta, Juliana Barchelli Pinheiro, Gilberto André e Silva, Viviane de Cássia Oliveira, A. G. Lourenço

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Abstract

Recent studies have identified inflammatory mediators as potential biomarkers for monitoring or diagnosing periodontitis. However, the brief half-life of these mediators, coupled with their variability among different individuals and across different stages of periodontal disease, may limit their reliability as biomarkers.In this study, we assessed the concentration profile of salivary biomarkers (IL-6, IL-8, and total protein) through repeated measurements within the same day and across different days in 79 patients exhibiting various states of periodontal health: intact periodontium, stable periodontitis, and active periodontitis. Additionally, we explored how daily variations, such as the interval between toothbrushing and eating, impact the levels of these salivary biomarkers and their diagnostic efficacy for periodontitis activity.Our results showed high salivary levels of IL-6 and total proteins in periodontitis patients (p < 0.001), with detection ability reflected by an Area Under the Receiver Operating Characteristic Curve (AUC-ROC) ranging between 0.709 and 0.852. Conversely, IL-8 levels were higher in patients with intact periodontium (p < 0.001), with an AUC-ROC for periodontitis detection between 0.671 and 0.815. Daily activities such as toothbrushing and eating influenced the levels of specific analytes, particularly total proteins (p < 0.001), but this did not affect their ability to detect periodontal disease activity. The highest measurement agreement, assessed by Intraclass Correlation Coefficients (ICC), was found for IL-6, with no significant differences in agreement between same-day and different-day measurements.Our study demonstrated consistency in the repeated measurements of salivary analytes, both within the same day and across different days, except for salivary total protein levels. These analytes exhibited variability within a range that did not undermine their effectiveness as biomarkers for periodontal disease.

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日常条件下唾液分析物的变化及其对牙周炎生物标记物的影响

最近的研究发现，炎症介质是监测或诊断牙周炎的潜在生物标志物。在本研究中，我们通过在同一天内和不同天内重复测量 79 名牙周健康状况不同的患者（牙周完好、稳定期牙周炎和活动期牙周炎）的唾液生物标志物（IL-6、IL-8 和总蛋白）的浓度曲线，对其进行了评估。结果显示，牙周炎患者唾液中的 IL-6 和总蛋白水平较高（p < 0.001），检测能力体现在接收者工作特征曲线下面积（AUC-ROC）介于 0.709 和 0.852 之间。相反，牙周完整的患者 IL-8 水平更高（p < 0.001），牙周炎检测的 AUC-ROC 在 0.671 和 0.815 之间。刷牙和进食等日常活动会影响特定分析物的水平，尤其是总蛋白（p < 0.001），但这并不影响其检测牙周疾病活动的能力。通过类内相关系数（ICC）评估，IL-6的测量一致性最高，同日测量和不同日测量的一致性无显著差异。这些分析物的变化范围不会影响其作为牙周病生物标志物的有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.