Overexpression of circular RNA hsa_circ_0008621 facilitates colorectal cancer progression and predicts poor prognosis

IF 2.9 4区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY

Annals of Gastroenterological Surgery Pub Date : 2024-03-22 DOI:10.1002/ags3.12793

Xiaohu Zhou, Lei Wu, Chunyan Tian

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Abstract

Aim

To evaluate the potential role of serum and tissue hsa_circ_0008621 as a prognostic biomarker for CRC patients. Focused on the functional role of hsa_circ_0008621 in colorectal cancer (CRC).

Methods

Serum and tissue hsa_circ_0008621 expression were quantified by qRT-PCR in 157 CRC patients, as well as 100 serums from healthy controls. Serum and tissue hsa_circ_0008621 expression was evaluated for their prognostic role in CRC patients using Kaplan–Meier curves and Multivariate Cox proportional hazards analysis. To further characterize the biological role of hsa_circ_0008621 expression in CRC, in vitro hsa_circ_0008621 inhibition was performed and the effects on cellular growth, migration, invasion, apoptosis, and glycolysis were explored. Next, the downstream molecules for hsa_circ_0008621 were predicted.

Results

Hsa_circ_0008621 expression was significantly upregulated in CRC tissues and serums. Serum hsa_circ_0008621 levels were significantly up-regulated in advanced-staged samples. High serum hsa_circ_0008621 expression was associated with shorter overall survival and recurrence-free survival in CRC patients. Multivariate Cox regression analysis identified a high level of serum hsa_circ_0008621 expression as an independent prognostic factor with respect to overall survival and recurrence-free survival. Loss of function assays for hsa_circ_0008621 in vitro led to a significant decrease in cell proliferation, migration, invasion, and glycolysis, but an increase in cell apoptosis. Hsa_circ_0008621 can sponge miR-532-5p, which targets SLC16A3.

Conclusion

High level of serum hsa_circ_0008621 is associated with poor survival in CRC and promotes CRC progression, suggesting it to be a promising non-invasive prognostic biomarker and novel therapeutic target in CRC patients.

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环状 RNA hsa_circ_0008621 的过表达可促进结直肠癌的进展并预测不良预后

评估血清和组织中 hsa_circ_0008621 作为 CRC 患者预后生物标志物的潜在作用。重点研究 hsa_circ_0008621 在结直肠癌（CRC）中的功能作用。通过 qRT-PCR 对 157 例 CRC 患者的血清和组织中 hsa_circ_0008621 的表达以及 100 例健康对照者的血清进行量化。利用 Kaplan-Meier 曲线和多变量 Cox 比例危险度分析评估了血清和组织中 hsa_circ_0008621 表达在 CRC 患者中的预后作用。为了进一步确定 hsa_circ_0008621 表达在 CRC 中的生物学作用，研究人员在体外对 hsa_circ_0008621 进行了抑制，并探讨了其对细胞生长、迁移、侵袭、凋亡和糖酵解的影响。接下来，研究人员预测了 hsa_circ_0008621 的下游分子。在晚期样本中，血清中的 hsa_circ_0008621 水平明显上调。血清中 hsa_circ_0008621 的高表达与 CRC 患者较短的总生存期和无复发生存期相关。多变量 Cox 回归分析发现，血清中 hsa_circ_0008621 的高表达水平是影响总生存期和无复发生存期的独立预后因素。体外hsa_circ_0008621功能缺失试验导致细胞增殖、迁移、侵袭和糖酵解显著减少，但细胞凋亡增加。血清中高水平的 hsa_circ_0008621 与 CRC 的低生存率有关，并会促进 CRC 的进展，这表明它是一种有前景的非侵入性预后生物标志物和 CRC 患者的新型治疗靶点。

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