Chemometric Tools to Characterize Phenolic Compounds with Antioxidant Activity of Melipona quadrifasciata Propolis from Brazil

IF 2.6 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2024-03-20 DOI:10.1007/s12161-024-02611-y

Bruno Henrique Fontoura, Ellen Cristina Perin, Anna Paula Simon, Celso Ferraz Bett, Priscila Rudiak Lustosa, Tatiane Luiza Cadorin Oldoni, Vanderlei Aparecido de Lima, José Abramo Marchese, Solange Teresinha Carpes

{"title":"Chemometric Tools to Characterize Phenolic Compounds with Antioxidant Activity of Melipona quadrifasciata Propolis from Brazil","authors":"Bruno Henrique Fontoura, Ellen Cristina Perin, Anna Paula Simon, Celso Ferraz Bett, Priscila Rudiak Lustosa, Tatiane Luiza Cadorin Oldoni, Vanderlei Aparecido de Lima, José Abramo Marchese, Solange Teresinha Carpes","doi":"10.1007/s12161-024-02611-y","DOIUrl":null,"url":null,"abstract":"<div>Propolis is an apicultural product whose composition depends on the climate conditions, geographical region, source of the resinous substance, and bee species. It is rich in phenolic compounds with antioxidant potential. This study aimed to establish better extraction conditions of bioactive compounds from Melipona quadrifasciata (MQ) propolis using a central composite rotational design (CCRD) and multivariate statistical tools. The solvent concentration and extraction time effects on the phenolic compounds profile with antioxidant activity (AA) were evaluated. The total phenolic compounds (TPC) and AA varied from 20.43 to 56.03 mg GAE g−1 (gallic acid equivalent) and 95.89 to 154.67 mmol Trolox g−1, respectively. The best condition for TPC and AA was 50% ethanol and 60 min of the extraction. However, each phenolic compound identified had a different best extraction condition, probably due to the molecules’ polarity and solvent interaction. The cinnamic acid was the highest content identified, followed by ferulic acid, gallic acid, epicatechin, p-coumaric acid, and catechin. Anyway, more extended times of extraction and solvent concentration closer to 50% ethanol can maximise the extraction of compounds with antioxidant potential. Thus, the Brazilian stingless bee propolis could be considered a potential source of bioactive compounds with potent antioxidant capacity, which could be further explored for future applications as natural antioxidants in several products.</div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"17 6","pages":"812 - 824"},"PeriodicalIF":2.6000,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Analytical Methods","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s12161-024-02611-y","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Propolis is an apicultural product whose composition depends on the climate conditions, geographical region, source of the resinous substance, and bee species. It is rich in phenolic compounds with antioxidant potential. This study aimed to establish better extraction conditions of bioactive compounds from Melipona quadrifasciata (MQ) propolis using a central composite rotational design (CCRD) and multivariate statistical tools. The solvent concentration and extraction time effects on the phenolic compounds profile with antioxidant activity (AA) were evaluated. The total phenolic compounds (TPC) and AA varied from 20.43 to 56.03 mg GAE g⁻¹ (gallic acid equivalent) and 95.89 to 154.67 mmol Trolox g⁻¹, respectively. The best condition for TPC and AA was 50% ethanol and 60 min of the extraction. However, each phenolic compound identified had a different best extraction condition, probably due to the molecules’ polarity and solvent interaction. The cinnamic acid was the highest content identified, followed by ferulic acid, gallic acid, epicatechin, p-coumaric acid, and catechin. Anyway, more extended times of extraction and solvent concentration closer to 50% ethanol can maximise the extraction of compounds with antioxidant potential. Thus, the Brazilian stingless bee propolis could be considered a potential source of bioactive compounds with potent antioxidant capacity, which could be further explored for future applications as natural antioxidants in several products.

Abstract Image

查看原文本刊更多论文

用化学计量学工具表征巴西 Melipona quadrifasciata 蜂胶中具有抗氧化活性的酚类化合物

蜂胶是一种养蜂产品，其成分取决于气候条件、地理区域、树脂物质来源和蜜蜂种类。它富含具有抗氧化潜力的酚类化合物。本研究旨在利用中央复合旋转设计（CCRD）和多元统计工具，建立从 Melipona quadrifasciata（MQ）蜂胶中提取生物活性化合物的更好条件。评估了溶剂浓度和提取时间对具有抗氧化活性（AA）的酚类化合物概况的影响。总酚化合物（TPC）和 AA 的变化范围分别为 20.43 至 56.03 mg GAE g-1（没食子酸当量）和 95.89 至 154.67 mmol Trolox g-1。萃取 TPC 和 AA 的最佳条件是 50% 的乙醇和 60 分钟的萃取时间。不过，每种酚类化合物的最佳萃取条件都不相同，这可能是由于分子的极性和溶剂相互作用造成的。肉桂酸的含量最高，其次是阿魏酸、没食子酸、表儿茶素、对香豆素和儿茶素。总之，延长萃取时间，使溶剂浓度接近 50%乙醇，可以最大限度地萃取出具有抗氧化潜力的化合物。因此，巴西无刺蜂胶可被视为一种具有强大抗氧化能力的生物活性化合物的潜在来源，未来可作为天然抗氧化剂进一步应用于多种产品中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.