Development and Validation of UPLC Tandem Mass Spectrometry Assay for Ceftibuten and Sulbactam in Human Plasma.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-01 DOI:10.1093/chromsci/bmae010

Karla Paulina Valero-Rivera, Martin Magaña-Aquino, María Del Carmen Romero-Méndez, Miriam Nayeli Morales-Barragán, Julia Sagahón-Azúa, Susanna Edith Medellín-Garibay, Rosa Del Carmen Milán-Segovia, Fidel Martínez-Gutiérrez, Silvia Romano-Moreno

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Abstract

A sensitive and rapid ultra-performance liquid chromatography coupled with -tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to determine ceftibuten (CTB) and sulbactam (SUL) in human plasma. An ACQUITY UPLC HSS T3 C18 (2.1 × 100 mm), 1.8 μm column with gradient elution of water (0.1% formic acid) and acetonitrile was used for separation at a flow rate of 0.2 mL/min. This method involves a simple sample preparation with acetonitrile. The calibration curves of CTB and SUL in plasma showed good linearity over the concentration range of 0.50-25 μg/mL and with a correlation coefficient (r2) >0.99. This method was validated in terms of selectivity, linearity, precision, accuracy and stability. High precision was obtained with coefficients of variation <15%. Excellent recovery in the range of 90-104% was achieved for CTB and SUL was 86-110%. The method has the potential utility to support pharmacometric modeling in clinical practice and biopharmaceutic studies.

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人血浆中头孢布坦和舒巴坦的 UPLC 串联质谱分析法的开发与验证

建立并验证了一种灵敏、快速的超高效液相色谱-串联质谱（UPLC-MS/MS）测定人体血浆中头孢布烯（CTB）和舒巴坦（SUL）的方法。采用ACQUITY UPLC HSS T3 C18 (2.1 × 100 mm), 1.8 μm色谱柱，以水（0.1%甲酸）和乙腈为流动相进行梯度洗脱，流速为0.2 mL/min。该方法只需用乙腈进行简单的样品制备。血浆中CTB和SUL的定标曲线在0.50-25 μg/mL浓度范围内线性关系良好，相关系数（r2）大于0.99。该方法在选择性、线性、精密度、准确度和稳定性方面都得到了验证。该方法的选择性、线性、精密度、准确度和稳定性都得到了验证。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.