Coptisine protects against transient focal cerebral ischaemic injury by regulation of arachidonic acid metabolism.

IF 2.8 4区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of Pharmacy and Pharmacology Pub Date : 2024-06-06 DOI:10.1093/jpp/rgae026

Junjie Zhang, Ao Qi, Jing Shen, Lulu Liu, Chun Cai, Hui Xu

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引用次数: 0

Abstract

Objectives: Coptisine (Cop), an alkaloid isolated from Rhizoma Coptidis, has a protective effect against central nervous system diseases such as cerebral ischaemia-reperfusion (IR). Dysregulations in fatty acids metabolism are associated with neuroprotection and neuroinflammation. However, the effect of Cop on fatty acids metabolomics during anti-IR remains unclear.

Methods: Cerebral IR rats were established by middle cerebral artery occlusion, and the therapeutic effect of Cop was evaluated by 2, 3, 5-triphenytetrazolium chloride staining and neurological deficits scores. By liquid chromatography-tandem mass spectrometry (LC-MS/MS), fatty acids metabolomics analysis in ischaemic hemisphere and serum were investigated.

Results: We observed Cop (2 mg/kg/qd) was able to reduce cerebral infarct size and ameliorate the neurological function score. Meanwhile decrease in tumour necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) after Cop treatment. Compared with control, down-regulation of cyclopentenone PGs (e.g., PGA2, PGJ2, and 15-deoxy- delta-12,14-PGJ2) was observed in cerebral IR, but upregulation of them when followed by Cop treatment. Similarly, we found the ratios of 14,15-dihydroxyeicosatrienoic acid(14,15-DHET)/arachidonic acid and 11,12-DHET/arachidonic acid was lower in cerebral IR injury relative to control, while their ratios were increased after Cop treatment.

Conclusion: Our results indicated that Cop protect against cerebral IR injury, and its mechanism might be closely associated with antiinflammation and the regulation of arachidonic acid metabolism.

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黄连素通过调节花生四烯酸代谢防止短暂性局灶性脑缺血损伤。

目的：从黄连中分离出的一种生物碱--黄连碱（Cop）对脑缺血再灌注等中枢神经系统疾病具有保护作用。脂肪酸代谢失调与神经保护和神经炎症有关。然而，Cop 对抗 IR 期间脂肪酸代谢组学的影响仍不清楚：方法：通过大脑中动脉闭塞建立脑 IR 大鼠，并通过 2，3，5-三苯基氯化四氮唑染色和神经功能缺损评分评估 Cop 的治疗效果。通过液相色谱-串联质谱法（LC-MS/MS），研究了缺血半球和血清中脂肪酸代谢组学分析：结果：我们观察到科普（2 毫克/千克/天）能够缩小脑梗塞面积，改善神经功能评分。同时，经 Cop 治疗后，肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）均有所下降。与对照组相比，环戊烯酮类 PGs（如 PGA2、PGJ2 和 15-脱氧-δ-12,14-PGJ2）在脑 IR 中出现下调，但在 Cop 治疗后出现上调。同样，我们发现在脑 IR 损伤中，14,15-二羟基二十碳三烯酸（14,15-DHET）/花生四烯酸和 11,12-DHET/ 花生四烯酸的比例相对于对照组较低，而在经过 Cop 治疗后，它们的比例上升：我们的研究结果表明，Cop 对脑 IR 损伤有保护作用，其机制可能与抗炎和调节花生四烯酸代谢密切相关。

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来源期刊

Journal of Pharmacy and Pharmacology 医学-药学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： JPP keeps pace with new research on how drug action may be optimized by new technologies, and attention is given to understanding and improving drug interactions in the body. At the same time, the journal maintains its established and well-respected core strengths in areas such as pharmaceutics and drug delivery, experimental and clinical pharmacology, biopharmaceutics and drug disposition, and drugs from natural sources. JPP publishes at least one special issue on a topical theme each year.