Interplay between diphenyl diselenide and copper: Impact on D. melanogaster survival, behavior, and biochemical parameters

IF 3.9 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2024-03-20 DOI:10.1016/j.cbpc.2024.109899

G.S. Rieder , T. Duarte , C.P. Delgado , A. Rodighiero , P.A. Nogara , L. Orian , M. Aschner , C.L. Dalla Corte , J.B.T. Da Rocha

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Abstract

Copper (Cu²⁺) is a biologically essential element that participates in numerous physiological processes. However, elevated concentrations of copper have been associated with cellular oxidative stress and neurodegenerative diseases. Organo‑selenium compounds such as diphenyl diselenide (DPDS) have in vitro and in vivo antioxidant properties. Hence, we hypothesized that DPDS may modulate the toxicity of Cu²⁺ in Drosophila melanogaster. The acute effects (4 days of exposure) caused by a high concentration of Cu²⁺ (3 mM) were studied using endpoints of toxicity such as survival and behavior in D. melanogaster. The potential protective effect of low concentration of DPDS (20 μM) against Cu²⁺ was also investigated. Adult flies aged 1–5 days post-eclosion (both sexes) were divided into four groups: Control, DPDS (20 μM), CuSO₄ (3 mM), and the combined exposure of DPDS (20 μM) and CuSO₄ (3 mM). Survival, biochemical, and behavioral parameters were determined. Co-exposure of DPDS and CuSO₄ increased acetylcholinesterase (AChE) activity and the generation of reactive oxygen species (ROS as determined by DFCH oxidation). Contrary to our expectation, the co-exposure reduced survival, body weight, locomotion, catalase activity, and cell viability in relation to control group. Taken together, DPDS potentiated the Cu²⁺ toxicity.

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二苯基二硒化物与铜之间的相互作用：对黑腹果蝇存活、行为和生化参数的影响

铜（Cu2+）是一种生物必需元素，参与多种生理过程。然而，铜浓度升高与细胞氧化应激和神经退行性疾病有关。二苯基二硒化物（DPDS）等有机硒化合物具有体外和体内抗氧化特性。因此，我们假设 DPDS 可调节 Cu2+ 对黑腹果蝇的毒性。我们利用黑腹果蝇的存活率和行为等毒性终点，研究了高浓度 Cu2+（3 mM）对黑腹果蝇的急性影响（暴露 4 天）。此外，还研究了低浓度 DPDS（20 μM）对 Cu2+ 的潜在保护作用。将孵化后 1-5 天的成蝇（雌雄均可）分为四组：对照组、DPDS（20 μM）组、CuSO4（3 mM）组、DPDS（20 μM）和 CuSO4（3 mM）联合暴露组。测定了存活率、生化指标和行为指标。同时暴露于 DPDS 和 CuSO4 会增加乙酰胆碱酯酶（AChE）的活性和活性氧（ROS，通过 DFCH 氧化测定）的生成。与我们的预期相反，与对照组相比，共同暴露降低了存活率、体重、运动能力、过氧化氢酶活性和细胞活力。综上所述，DPDS增强了Cu2+的毒性。

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来源期刊

Comparative Biochemistry and Physiology C-toxicology & Pharmacology 医学-动物学

CiteScore

7.50

自引率

5.10%

发文量

206

审稿时长

30 days

期刊介绍： Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.