Diverse models of cavity engineering in enzyme modification: Creation, filling, and reshaping

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances Pub Date : 2024-03-20 DOI:10.1016/j.biotechadv.2024.108346

Zehua Zhang , Yongchao Cai , Nan Zheng , Yu Deng , Ling Gao , Qiong Wang , Xiaole Xia

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引用次数: 0

Abstract

Most enzyme modification strategies focus on designing the active sites or their surrounding structures. Interestingly, a large portion of the enzymes (60%) feature active sites located within spacious cavities. Despite recent discoveries, cavity-mediated enzyme engineering remains crucial for enhancing enzyme properties and unraveling folding-unfolding mechanisms. Cavity engineering influences enzyme stability, catalytic activity, specificity, substrate recognition, and docking. This article provides a comprehensive review of various cavity engineering models for enzyme modification, including cavity creation, filling, and reshaping. Additionally, it also discusses feasible tools for geometric analysis, functional assessment, and modification of cavities, and explores potential future research directions in this field. Furthermore, a promising universal modification strategy for cavity engineering that leverages state-of-the-art technologies and methodologies to tailor cavities according to the specific requirements of industrial production conditions is proposed.

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酶改性空腔工程的多种模式：创建、填充和重塑。

大多数酶修饰策略都侧重于设计活性位点或其周围结构。有趣的是，很大一部分酶（60%）的活性位点位于宽敞的空腔内。尽管最近有了新发现，但空腔介导的酶工程对于增强酶的特性和揭示折叠-解折机制仍然至关重要。空腔工程影响着酶的稳定性、催化活性、特异性、底物识别和对接。本文全面综述了用于酶修饰的各种空腔工程模型，包括空腔的创建、填充和重塑。此外，文章还讨论了对空腔进行几何分析、功能评估和修饰的可行工具，并探讨了这一领域未来潜在的研究方向。此外，报告还提出了一种前景广阔的空腔工程通用改性策略，该策略可利用最先进的技术和方法，根据工业生产条件的具体要求定制空腔。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.