Liquid biopsy of wash samples obtained via endoscopic ultrasound-guided fine-needle biopsy: Comparison with liquid biopsy of plasma in pancreatic cancer

IF 1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Diagnostic Cytopathology Pub Date : 2024-03-22 DOI:10.1002/dc.25306

Hiroshi Ohyama MD, PhD, Yosuke Hirotsu PhD, Kenji Amemiya MS, Hiroyuki Amano MD, Sumio Hirose MD, PhD, Toshio Oyama MD, PhD, Yuji Iimuro MD, PhD, Yuichiro Kojima MD, PhD, Rintaro Mikata MD, PhD, Hitoshi Mochizuki MD, PhD, Naoya Kato MD, PhD, Masao Omata MD, PhD

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引用次数: 0

Abstract

Objectives

Pancreatic cancer (PC) has a poor prognosis and limited treatment options. Liquid biopsy, which analyzes circulating tumor DNA (ctDNA) in blood, holds promise for precision medicine; however, low ctDNA detection rates pose challenges. This study aimed to investigate the utility of wash samples obtained via endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) as a liquid biopsy for PC.

Methods

A total of 166 samples (42 formalin-fixed paraffin-embedded [FFPE] tissues, 80 wash samples, and 44 plasma samples) were collected from 48 patients with PC for genomic analysis. DNA was extracted and quantified, and 60 significantly mutated genes were sequenced. The genomic profiles of FFPE tissues, wash samples, and plasma samples were compared. Finally, the ability to detect druggable mutations in 80 wash samples and 44 plasma samples was investigated.

Results

The amount of DNA was significantly lower in plasma samples than in wash samples. Genomic analysis revealed a higher detection rate of oncogenic mutations in FFPE tissues (98%) and wash samples (96%) than in plasma samples (18%) and a comparable detection rate in FFPE tissues and wash samples. Tumor-derived oncogenic mutations were detected more frequently in wash samples than in plasma samples. Furthermore, the oncogenic mutations detection rate remained high in wash samples at all PC stages but low in plasma samples even at advanced PC stages. Using wash samples was more sensitive than plasma samples for identifying oncogenic and druggable mutations.

Conclusions

The wash sample obtained via EUS-FNB is an ideal specimen for use as a liquid biopsy for PC.

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通过内窥镜超声引导细针活检获得的洗液样本的液体活检：与胰腺癌血浆液体活检的比较。

目的：胰腺癌（PC）预后不良，治疗方案有限。液体活检可分析血液中的循环肿瘤DNA（ctDNA），有望实现精准医疗；然而，ctDNA的低检出率带来了挑战。本研究旨在探讨通过内镜超声引导下细针活检（EUS-FNB）获得的清洗样本作为PC液体活检的实用性：方法：从48名PC患者中收集了166份样本（42份福尔马林固定石蜡包埋组织、80份清洗样本和44份血浆样本）进行基因组分析。对 DNA 进行了提取和定量，并对 60 个明显突变的基因进行了测序。对 FFPE 组织、清洗样本和血浆样本的基因组图谱进行了比较。最后，研究了在 80 份清洗样本和 44 份血浆样本中检测药物突变的能力：结果：血浆样本中的 DNA 量明显低于清洗样本。基因组分析显示，FFPE 组织（98%）和清洗样本（96%）的致癌突变检出率高于血浆样本（18%），FFPE 组织和清洗样本的检出率相当。与血浆样本相比，清洗样本中更常检测到来自肿瘤的致癌突变。此外，在所有 PC 阶段，清洗样本的致癌突变检出率都很高，但即使在 PC 晚期，血浆样本的致癌突变检出率也很低。在确定致癌突变和可治疗突变方面，清洗样本比血浆样本更敏感：结论：通过 EUS-FNB 获得的冲洗样本是用作 PC 液体活检的理想样本。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic Cytopathology 医学-病理学

CiteScore

2.60

自引率

7.70%

发文量

163

审稿时长

3-6 weeks

期刊介绍： Diagnostic Cytopathology is intended to provide a forum for the exchange of information in the field of cytopathology, with special emphasis on the practical, clinical aspects of the discipline. The editors invite original scientific articles, as well as special review articles, feature articles, and letters to the editor, from laboratory professionals engaged in the practice of cytopathology. Manuscripts are accepted for publication on the basis of scientific merit, practical significance, and suitability for publication in a journal dedicated to this discipline. Original articles can be considered only with the understanding that they have never been published before and that they have not been submitted for simultaneous review to another publication.