Interference between Escherichia coli genotypes from the E. coli peritonitis syndrome given simultaneously to productive SPF White Leghorn hens by intratracheal inoculation.

IF 2.5 2区农林科学 Q1 VETERINARY SCIENCES

Avian Pathology Pub Date : 2024-10-01 Epub Date: 2024-04-11 DOI:10.1080/03079457.2024.2334683

W J M Landman, J H H van Eck, A E Heuvelink

{"title":"Interference between Escherichia coli genotypes from the E. coli peritonitis syndrome given simultaneously to productive SPF White Leghorn hens by intratracheal inoculation.","authors":"W J M Landman, J H H van Eck, A E Heuvelink","doi":"10.1080/03079457.2024.2334683","DOIUrl":null,"url":null,"abstract":"The purpose of the present study was to examine if potentiation of mortality occurred after simultaneous administration of several Escherichia coli genotypes, each capable of inducing the E. coli peritonitis syndrome, in comparison with single genotype application. Five groups of productive specified pathogen free White Leghorn hens were housed in isolators. Groups 1-4 consisted of 32 hens each, group 5 of 10 hens. At 32 weeks of age all groups were inoculated intratracheally. Groups 1 and 2 were inoculated with a mix of four E. coli genotypes and groups 3 and 4 with a mix of four other genotypes. Groups 1 and 3 were given 1 median lethal dose (LD50) of each genotype per hen and groups 2 and 4 had a dose of 0.1 LD50 per genotype per hen; group 5 was mock inoculated. The experiment ended one week after inoculations. In Group 5, no mortality occurred and gross lesions were absent at post-mortem examination. Mortality in groups 1 and 3 was 84% and 81%, respectively; in groups 2 and 4 59% and 66%, respectively. Although mortality in groups 1 and 3 exceeded the expected 50%, this could not be due to potentiation as cluster analysis of reisolates showed that in individual hens only one genotype was found, indicating interference between E. coli genotypes. In groups all four or only two genotypes were recovered, showing that not all genotypes will induce colibacillosis in all experimental groups. Therefore, broad protection can be best assessed by challenging with various single genotypes.RESEARCH HIGHLIGHTS All four or only two E. coli genotypes were found in groups of hens given mixes of four genotypes.In contrast, only one genotype was found in individual hens.E. coli genotypes interfere with each other in hens after given as a mix.Interference is likely based on a random process.Broad protection can best be assessed by challenging with single genotypes.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Avian Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/03079457.2024.2334683","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/4/11 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

The purpose of the present study was to examine if potentiation of mortality occurred after simultaneous administration of several Escherichia coli genotypes, each capable of inducing the E. coli peritonitis syndrome, in comparison with single genotype application. Five groups of productive specified pathogen free White Leghorn hens were housed in isolators. Groups 1-4 consisted of 32 hens each, group 5 of 10 hens. At 32 weeks of age all groups were inoculated intratracheally. Groups 1 and 2 were inoculated with a mix of four E. coli genotypes and groups 3 and 4 with a mix of four other genotypes. Groups 1 and 3 were given 1 median lethal dose (LD₅₀) of each genotype per hen and groups 2 and 4 had a dose of 0.1 LD₅₀ per genotype per hen; group 5 was mock inoculated. The experiment ended one week after inoculations. In Group 5, no mortality occurred and gross lesions were absent at post-mortem examination. Mortality in groups 1 and 3 was 84% and 81%, respectively; in groups 2 and 4 59% and 66%, respectively. Although mortality in groups 1 and 3 exceeded the expected 50%, this could not be due to potentiation as cluster analysis of reisolates showed that in individual hens only one genotype was found, indicating interference between E. coli genotypes. In groups all four or only two genotypes were recovered, showing that not all genotypes will induce colibacillosis in all experimental groups. Therefore, broad protection can be best assessed by challenging with various single genotypes.RESEARCH HIGHLIGHTS All four or only two E. coli genotypes were found in groups of hens given mixes of four genotypes.In contrast, only one genotype was found in individual hens.E. coli genotypes interfere with each other in hens after given as a mix.Interference is likely based on a random process.Broad protection can best be assessed by challenging with single genotypes.

查看原文本刊更多论文

通过气管内接种法同时给高产 SPF 白乐鸡注射大肠杆菌腹膜炎综合征的大肠杆菌基因型之间的干扰。

本研究的目的是，与单一基因型的应用相比，研究同时应用几种大肠杆菌基因型（每种基因型都能诱发大肠杆菌腹膜炎综合征）是否会增加死亡率。在隔离器中饲养了五组无特定病原体的高产白 Leghorn 母鸡。1-4 组各有 32 只母鸡，第 5 组有 10 只母鸡。32 周龄时，对所有组别进行气管内接种。第 1 组和第 2 组混合接种 4 种基因型的大肠杆菌，第 3 组和第 4 组混合接种 4 种其他基因型的大肠杆菌。第 1 组和第 3 组给每只母鸡每种基因型 1 个中位致死剂量（LD50），第 2 组和第 4 组给每只母鸡每种基因型 0.1 个中位致死剂量。第 5 组为模拟接种。实验在接种一周后结束。第 5 组未出现死亡，死后检查也未发现严重病变。第 1 组和第 3 组的死亡率分别为 84% 和 81%；第 2 组和第 4 组的死亡率分别为 59% 和 66%。虽然第 1 组和第 3 组的死亡率超过了预期的 50%，但这不可能是由于增效作用造成的，因为对再分离物的聚类分析显示，在单只母鸡中只发现了一种基因型，这表明大肠杆菌基因型之间存在干扰。在实验组中，全部四种基因型或只有两种基因型被发现，这表明并非所有基因型都会在所有实验组中诱发大肠杆菌病。研究要点在混合饲喂 4 种基因型的母鸡组中，发现了所有 4 种或 2 种大肠杆菌基因型，而在母鸡个体中只发现了一种基因型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Avian Pathology 农林科学-兽医学

CiteScore

4.50

自引率

10.70%

发文量

审稿时长

1 months

期刊介绍： Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.