External quality control survey on identification of microorganisms using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Kazuyuki Sogawa, Azumi Fujinaga, Hajime Okumura, Makiko Kiyosuke, Syota Murata, Koji Kusaba, Kohei Uechi, Kazuki Horiuchi, Kazunari Yasuda, Masami Murakami, Tomohiro Nakayama
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Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry( MALDI-TOF MS) is a bacterial typing tool that was approved as a medical device in 2011. However, external accuracy control examination of bacterial typing using mass spectrometry is still only performed on a small scale. In this study, E. faecium and S. maltophilia were selected and tested according to established procedures using Score Values at 228 institutions. The Score Values for MALDI Biotyper were 2.43±0.08 for E. faecium and 2.38±0.08 for S. maltophilia; and those for VITEK MS/PRIME were 99.9±0.0 for E. faecium and S. maltophilia. These results suggest that it is useful to evaluate external accuracy control with Score Values using the procedures we have developed.

利用基质辅助激光解吸电离飞行时间质谱法鉴定微生物的外部质量控制调查。
基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)是一种细菌分型工具,于2011年被批准为医疗设备。是一种细菌分型工具,2011 年被批准为医疗设备。然而,利用质谱进行细菌分型的外部准确性控制检查仍只在小范围内进行。在本研究中,228 家机构按照既定程序使用评分值对粪肠球菌和嗜麦芽糖酵母菌进行了筛选和检测。MALDI Biotyper 的评分值为 2.43±0.08(粪大肠杆菌)和 2.38±0.08(嗜麦芽糖酵母菌);VITEK MS/PRIME 的评分值为 99.9±0.0(粪大肠杆菌)和 99.9±0.0(嗜麦芽糖酵母菌)。这些结果表明,使用我们开发的程序来评估带有评分值的外部准确度控制是非常有用的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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