Antimicrobial resistance genetic determinants and susceptibility profile of Pseudomonas aeruginosa isolated from clinical samples in a tertiary hospital in Ogun State, Nigeria.

IF 1.9 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2024-07-05 DOI:10.1093/trstmh/trae012

Oluwatoyin Olawunmi Adeyelu, Edidiong Nkiruka Essien, Valentine Adebote, Abraham Ajayi, Utibeima Udo Essiet, Adeyemi Isaac Adeleye, Stella Ifeanyi Smith

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Abstract

Background: Genetic determinants are known to promote antibiotic resistance through horizontal gene transfer.

Methods: We molecularly characterized integrons, plasmid replicon types and metallo-β-lactamase-encoding genes of 38 Pseudomonas aeruginosa strains isolated from clinical samples.

Results: The P. aeruginosa isolates displayed high resistance (97.4%) to β-lactams. Seventeen (44.74%) of them possessed plasmids. Of the 17 isolates that possessed plasmids, 11 (64.7%) of them harboured IncFIA plasmid replicon type, while 6 (35.3%), 5 (29.4%) and 5 (29.4%) were of the IncFIB, IncF and IncW types, respectively. The intI1 gene was detected in 19 (50%) of the isolates. The blaNDM-A, blaNDM-B and blaVIM genes were detected in 14 (35.9%), 4 (10.3%) and 5 (12.8%) of the isolates, respectively.

Conclusions: High resistance to β-lactams was observed among P. aeruginosa strains of clinical origin in this study. They possessed transmissible genetic elements indicating the potential for continuous dissemination, thus continuous surveillance is advocated.

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从尼日利亚奥贡州一家三级医院的临床样本中分离出的铜绿假单胞菌的抗菌药耐药性基因决定因素和药敏性概况。

背景方法：我们对从临床样本中分离出的 38 株铜绿假单胞菌的整合子、质粒复制子类型和金属-β-内酰胺酶编码基因进行了分子鉴定：我们对从临床样本中分离出的38株铜绿假单胞菌的整合子、质粒复制子类型和金属β-内酰胺酶编码基因进行了分子鉴定：结果：铜绿假单胞菌分离株对β-内酰胺类药物的耐药性较高（97.4%）。其中 17 株（44.74%）具有质粒。在 17 个具有质粒的分离物中，11 个（64.7%）具有 IncFIA 质粒复制子类型，6 个（35.3%）、5 个（29.4%）和 5 个（29.4%）分别具有 IncFIB、IncF 和 IncW 类型。在 19 个（50%）分离物中检测到 intI1 基因。在 14 个（35.9%）、4 个（10.3%）和 5 个（12.8%）分离物中分别检测到 blaNDM-A、blaNDM-B 和 blaVIM 基因：结论：本研究观察到临床来源的铜绿假单胞菌菌株对β-内酰胺类药物具有高度耐药性。结论：本研究观察到临床来源的铜绿假单胞菌株对β-内酰胺类药物有很高的耐药性，它们具有可传播的遗传因子，表明有持续传播的潜力，因此建议进行持续监测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transactions of The Royal Society of Tropical Medicine and Hygiene 医学-公共卫生、环境卫生与职业卫生

CiteScore

4.00

自引率

9.10%

发文量

115

审稿时长

4-8 weeks

期刊介绍： Transactions of the Royal Society of Tropical Medicine and Hygiene publishes authoritative and impactful original, peer-reviewed articles and reviews on all aspects of tropical medicine.