FVB/NJ strain as a mouse model for cutaneous leishmaniasis by Leishmania (L.) amazonensis.

IF 2.5 4区医学 Q2 PARASITOLOGY

Memorias do Instituto Oswaldo Cruz Pub Date : 2024-03-15 eCollection Date: 2024-01-01 DOI:10.1590/0074-02760230182

Guilherme Moreira Paiva Carrara, Beatriz Simonsen Stolf

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引用次数: 0

Abstract

Background: Leishmaniases encompass a spectrum of neglected diseases caused by parasites of the genus Leishmania, grouped in two forms: tegumentary and visceral leishmaniasis.

Objectives: In this study, we propose Friend Virus B NIH Jackson (FVB/NJ) mouse strain as a new experimental model of infection with Leishmania (Leishmania) amazonensis, the second most prevalent agent of tegumentary leishmaniasis in Brazil.

Methods and findings: We performed in vitro infections of FVB/NJ macrophages and compared them with BALB/c macrophages, showing that BALB/c cells have higher infection percentages and a higher number of amastigotes/cell. Phagocytosis assays indicated that BALB/c and FVB/NJ macrophages have similar capacity to uptake parasites after 5 min incubations. We also investigated promastigotes' resistance to sera from FVB/NJ and BALB/c and observed no difference between the two sera, even though FVB/NJ has a deficiency in complement components. Finally, we subcutaneously infected FVB/NJ and BALB/c mice with 2 × 106 parasites expressing luciferase. Analysis of lesion development for 12 weeks showed that FVB/NJ and BALB/c mice have similar lesion profiles and parasite burdens.

Main conclusions: This work characterises for the first time the FVB/NJ mouse as a new model for tegumentary leishmaniasis caused by Leishmania (L.) amazonensis.

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将 FVB/NJ 株作为亚马逊利什曼病皮肤利什曼病的小鼠模型。

背景：利什曼病是由利什曼原虫引起的一种被忽视的疾病：利什曼病包括由利什曼属寄生虫引起的一系列被忽视的疾病，分为两种形式：皮肤利什曼病和内脏利什曼病：在本研究中，我们建议将 Friend Virus B NIH Jackson（FVB/NJ）小鼠品系作为感染亚马逊利什曼病（Leishmania）的新实验模型，亚马逊利什曼病是巴西第二大皮损利什曼病病原体：我们对 FVB/NJ 巨噬细胞进行了体外感染，并与 BALB/c 巨噬细胞进行了比较，结果表明 BALB/c 细胞的感染率更高，非主原体/细胞的数量也更多。吞噬试验表明，BALB/c 和 FVB/NJ 巨噬细胞在培养 5 分钟后摄取寄生虫的能力相似。我们还研究了原虫对FVB/NJ和BALB/c血清的抗性，观察到这两种血清之间没有差异，尽管FVB/NJ缺乏补体成分。最后，我们用 2 × 106 只表达荧光素酶的寄生虫皮下注射感染 FVB/NJ 和 BALB/c 小鼠。对 12 周病变发展的分析表明，FVB/NJ 和 BALB/c 小鼠的病变情况和寄生虫负担相似：主要结论：这项研究首次描述了 FVB/NJ 小鼠作为亚马逊利什曼病引起的皮肤利什曼病新模型的特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Memorias do Instituto Oswaldo Cruz 医学-寄生虫学

CiteScore

5.00

自引率

3.60%

发文量

审稿时长

3-8 weeks

期刊介绍： Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.