Activities of pumpkin seed oil against Biomphalaria alexandrina snails and the infective stages of Schistosoma mansoni with special emphasis on genotoxic and histopathological alterations.

IF 1.3 3区 生物学 Q4 PARASITOLOGY
S E Mohammed, H S Mossalem, R M Gad El-Karim, A T Morsy, A M Ammar
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Abstract

Schistosomiasis is a serious health issue in tropical regions, and natural compounds have gained popularity in medical science. This study investigated the potential effects of pumpkin seed oil (PSO) on Biomphalaria [B.] alexandrina snails (Ehrenberg, 1831), Schistosoma [S.] mansoni (Sambon, 1907) miracidium, and cercariae. The chemical composition of PSO was determined using gas chromatography/mass spectrometry. A bioassay was performed to evaluate the effects of PSO on snails, miracidia, and cercariae. The results showed no significant mortality of B. alexandrina snails after exposure to PSO, but it caused morphological changes in their hemocytes at 1.0 mg/ml for 24 hours. PSO exhibited larvicidal activity against miracidia after 2 hours of exposure at a LC50 of 618.4 ppm. A significant increase in the mortality rate of miracidia was observed in a dose- and time-dependent manner, reaching a 100% death rate after 10 minutes at LC90 and 15 minutes at LC50 concentration. PSO also showed effective cercaricidal activity after 2 hours of exposure at a LC50 of 290.5 ppm. Histological examination revealed multiple pathological changes in the digestive and hermaphrodite glands. The PSO had genotoxic effects on snails, which exhibited a significant increase [p≤0.05] in comet parameters compared to the control. The findings suggest that PSO has potential as a molluscicide, miracidicide, and cercaricide, making it a possible alternative to traditional molluscicides in controlling schistosomiasis.

南瓜籽油对Biomphalaria alexandrina蜗牛和曼氏血吸虫感染阶段的活性,特别强调基因毒性和组织病理学改变。
血吸虫病是热带地区的一个严重健康问题,天然化合物在医学科学中越来越受欢迎。本研究调查了南瓜籽油(PSO)对 Bomphalaria [B.] alexandrina 蜗牛(Ehrenberg,1831 年)、Schistosoma [S.] mansoni(Sambon,1907 年)蜃和carcariae 的潜在影响。使用气相色谱/质谱法测定了 PSO 的化学成分。进行了生物测定,以评估 PSO 对蜗牛、弧蝽和蛔虫的影响。结果表明,接触 PSO 24 小时后,B. alexandrina 蜗牛没有明显死亡,但 1.0 毫克/毫升的 PSO 会导致其血细胞形态发生变化。接触 PSO 2 小时后,LC50 为 618.4 ppm,PSO 对螨类具有杀幼虫活性。在 LC90 浓度和 LC50 浓度下,弧菌死亡率分别在 10 分钟和 15 分钟后达到 100%。在半数致死浓度为百万分之 290.5 时,接触 PSO 2 小时后也显示出有效的杀螨活性。组织学检查显示,消化腺和雌雄同体腺发生了多种病理变化。PSO 对蜗牛有遗传毒性作用,与对照组相比,彗星参数显著增加 [p≤0.05]。研究结果表明,PSO 具有作为杀软体动物剂、杀螨剂和杀螨剂的潜力,可替代传统的杀软体动物剂来控制血吸虫病。
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来源期刊
Journal of Helminthology
Journal of Helminthology 生物-动物学
CiteScore
2.80
自引率
12.50%
发文量
127
审稿时长
3 months
期刊介绍: Journal of Helminthology publishes original papers and review articles on all aspects of pure and applied helminthology, particularly those helminth parasites of environmental health, medical or veterinary importance. Research papers on helminths in wildlife hosts, including plant and insect parasites, are also published along with taxonomic papers contributing to the systematics of a group. The journal will be of interest to academics and researchers involved in the fields of human and veterinary parasitology, public health, microbiology, ecology and biochemistry.
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