Effects of the application of low-temperature atmospheric plasma on titanium implants on wound healing in peri-implant connective tissue in rats.

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International Journal of Implant Dentistry Pub Date : 2024-03-21 DOI:10.1186/s40729-024-00524-3

Atsuro Harada, Hodaka Sasaki, Yosuke Asami, Kiyotoshi Hanazawa, Sota Miyazaki, Hideshi Sekine, Yasutomo Yajima

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引用次数: 0

Abstract

Purpose: This study aimed to clarify the effects of surface modification of titanium (Ti) implants by low-temperature atmospheric pressure plasma treatment on wound healing and cell attachment for biological sealing in peri-implant soft tissue.

Methods: Hydrophilization to a Ti disk using a handheld low-temperature atmospheric pressure plasma device was evaluated by a contact angle test and compared with an untreated group. In in vivo experiments, plasma-treated pure Ti implants using a handheld plasma device (experimental group: PL) and untreated implants (control group: Cont) were placed into the rat upper molar socket, and samples were harvested at 3, 7 and 14 days after surgery. Histological evaluation was performed to assess biological sealing, collagen- and cell adhesion-related gene expression by reverse transcription quantitative polymerase chain reaction, collagen fiber detection by Picrosirius Red staining, and immunohistochemistry for integrins.

Results: In in vivo experiments, increased width of the peri-implant connective tissue (PICT) and suppression of epithelial down growth was observed in PL compared with Cont. In addition, high gene expression of types I and XII collagen at 7 days and acceleration of collagen maturation was recognized in PL. Strong immunoreaction of integrin α2, α5, and β1 was observed at the implant contact area of PICT in PL.

Conclusions: The handheld low-temperature atmospheric pressure plasma device provided hydrophilicity on the Ti surface and maintained the width of the contact area of PICT to the implant surface as a result of accelerated collagen maturation and fibroblast adhesion, compared to no plasma application.

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在钛种植体上应用低温大气等离子体对大鼠种植体周围结缔组织伤口愈合的影响。

目的：本研究旨在阐明通过低温常压等离子体处理对钛（Ti）种植体进行表面改性对伤口愈合和细胞附着的影响，以实现种植体周围软组织的生物密封。方法：通过接触角测试评估使用手持式低温常压等离子体装置对钛盘的亲水性，并与未处理组进行比较。在体内实验中，将使用手持式等离子装置进行等离子处理的纯钛种植体（实验组：PL）和未经处理的种植体（对照组：Cont）植入大鼠上臼齿窝，并在术后 3、7 和 14 天采集样本。通过逆转录定量聚合酶链式反应评估生物密封性、胶原蛋白和细胞粘附相关基因的表达、毕克休斯红染色检测胶原纤维以及免疫组化检测整合素，并进行组织学评估：在体内实验中，与 Cont 相比，PL 的种植体周围结缔组织（PICT）宽度增加，上皮向下生长受到抑制。此外，在 PL 中，7 天时 I 型和 XII 型胶原蛋白的基因表达量较高，胶原蛋白成熟速度加快。在 PL 的 PICT 种植体接触区观察到整合素 α2、α5 和 β1 的强烈免疫反应：结论：与不应用等离子体相比，手持式低温常压等离子体装置可提供钛表面的亲水性，并通过加速胶原蛋白成熟和成纤维细胞粘附来保持 PICT 与种植体表面接触区域的宽度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Implant Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

1.70

自引率

7.40%

发文量

审稿时长

13 weeks

期刊介绍： The International Journal of Implant Dentistry is a peer-reviewed open access journal published under the SpringerOpen brand. The journal is dedicated to promoting the exchange and discussion of all research areas relevant to implant dentistry in the form of systematic literature or invited reviews, prospective and retrospective clinical studies, clinical case reports, basic laboratory and animal research, and articles on material research and engineering.