Brain inflammation in experimental autoimmune encephalomyelitis induced in Dark Agouti rats with spinal cord homogenate

IF 3.3 4区医学 Q3 IMMUNOLOGY

Immunology letters Pub Date : 2024-03-19 DOI:10.1016/j.imlet.2024.106852

Goran Stegnjaić , Bojan Jevtić , Milica Lazarević , Đurđica Ignjatović , Mirko Tomić , Neda Nikolovski , Ivana Bjelobaba , Miljana Momčilović , Mirjana Dimitrijević , Đorđe Miljković , Suzana Stanisavljević

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引用次数: 0

Abstract

We have recently characterized experimental autoimmune encephalomyelitis (EAE) induced in DA rats with spinal cord homogenate without complete Freund's adjuvant (CFA). The main advantage of this multiple sclerosis model is the lack of CFA-related confounding effects which represent the major obstacles in translating findings from EAE to multiple sclerosis. Here, antigen specificity of the cellular and humoral immune response directed against the central nervous system was explored. The reactivity of T and B cells to myelin basic protein, myelin oligodendrocyte glycoprotein, and β-synuclein was detected. Having in mind that reactivity against β-synuclein was previously associated with autoimmunity against the brain, the infiltration of immune cells into different brain compartments, i.e. pons, cerebellum, hippocampus, and cortex was determined. T cell infiltration was observed in all structures examined. This finding stimulated investigation of the effects of immunization on DA rat behavior using the elevated plus maze and the open field test. Rats recovered from EAE displayed increased anxiety-like behavior. These data support CFA-free EAE in DA rats as a useful model for multiple sclerosis research.

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用脊髓匀浆诱导黑暗阿古提大鼠患上实验性自身免疫性脑脊髓炎的脑部炎症。

最近，我们研究了用脊髓匀浆诱导的 DA 大鼠实验性自身免疫性脑脊髓炎（EAE）的特点，这种诱导方法不使用完全弗氏佐剂（CFA）。这种多发性硬化症模型的主要优点是缺乏与 CFA 相关的混杂效应，而这些混杂效应是将 EAE 研究结果转化为多发性硬化症的主要障碍。本文探讨了针对中枢神经系统的细胞和体液免疫反应的抗原特异性。研究人员检测了 T 细胞和 B 细胞对髓鞘碱性蛋白、髓鞘少突胶质细胞糖蛋白和β-突触核蛋白的反应性。考虑到对β-突触核蛋白的反应性以前与脑自身免疫有关，因此测定了免疫细胞对不同脑区（即脑桥、小脑、海马和皮层）的浸润情况。在所有受检结构中都观察到了 T 细胞浸润。这一发现促使研究人员使用高架加迷宫和开阔地试验研究免疫对 DA 大鼠行为的影响。从 EAE 中恢复的大鼠表现出更多的焦虑样行为。这些数据支持将 DA 大鼠无 CFA EAE 作为一种有用的多发性硬化症研究模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunology letters 医学-免疫学

CiteScore

7.60

自引率

0.00%

发文量

审稿时长

44 days

期刊介绍： Immunology Letters provides a vehicle for the speedy publication of experimental papers, (mini)Reviews and Letters to the Editor addressing all aspects of molecular and cellular immunology. The essential criteria for publication will be clarity, experimental soundness and novelty. Results contradictory to current accepted thinking or ideas divergent from actual dogmas will be considered for publication provided that they are based on solid experimental findings. Preference will be given to papers of immediate importance to other investigators, either by their experimental data, new ideas or new methodology. Scientific correspondence to the Editor-in-Chief related to the published papers may also be accepted provided that they are short and scientifically relevant to the papers mentioned, in order to provide a continuing forum for discussion.