Metal and oxidative potential exposure through particle inhalation and oxidative stress biomarkers: a 2-week pilot prospective study among Parisian subway workers.

IF 2.6 3区医学 Q2 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

International Archives of Occupational and Environmental Health Pub Date : 2024-05-01 Epub Date: 2024-03-20 DOI:10.1007/s00420-024-02054-2

Jean-Jacques Sauvain, Maud Hemmendinger, Thomas Charreau, Valérie Jouannique, Amélie Debatisse, Guillaume Suárez, Nancy B Hopf, Irina Guseva Canu

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引用次数: 0

Abstract

Objective: In this pilot study on subway workers, we explored the relationships between particle exposure and oxidative stress biomarkers in exhaled breath condensate (EBC) and urine to identify the most relevant biomarkers for a large-scale study in this field.

Methods: We constructed a comprehensive occupational exposure assessment among subway workers in three distinct jobs over 10 working days, measuring daily concentrations of particulate matter (PM), their metal content and oxidative potential (OP). Individual pre- and post-shift EBC and urine samples were collected daily. Three oxidative stress biomarkers were measured in these matrices: malondialdehyde (MDA), 8-hydroxy-2'deoxyguanosine (8-OHdG) and 8-isoprostane. The association between each effect biomarker and exposure variables was estimated by multivariable multilevel mixed-effect models with and without lag times.

Results: The OP was positively associated with Fe and Mn, but not associated with any effect biomarkers. Concentration changes of effect biomarkers in EBC and urine were associated with transition metals in PM (Cu and Zn) and furthermore with specific metals in EBC (Ba, Co, Cr and Mn) and in urine (Ba, Cu, Co, Mo, Ni, Ti and Zn). The direction of these associations was both metal- and time-dependent. Associations between Cu or Zn and MDA_EBC generally reached statistical significance after a delayed time of 12 or 24 h after exposure. Changes in metal concentrations in EBC and urine were associated with MDA and 8-OHdG concentrations the same day.

Conclusion: Associations between MDA in both EBC and urine gave opposite response for subway particles containing Zn versus Cu. This diverting Zn and Cu pattern was also observed for 8-OHdG and urinary concentrations of these two metals. Overall, MDA and 8-OHdG responses were sensitive for same-day metal exposures in both matrices. We recommend MDA and 8-OHdG in large field studies to account for oxidative stress originating from metals in inhaled particulate matter.

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通过吸入颗粒接触金属和氧化潜能以及氧化应激生物标志物：对巴黎地铁工人进行的为期两周的前瞻性试点研究。

研究目的在这项针对地铁工作人员的试点研究中，我们探讨了颗粒物暴露与呼出气体冷凝物（EBC）和尿液中氧化应激生物标志物之间的关系，以便为该领域的大规模研究确定最相关的生物标志物：我们对从事三种不同工作的地铁工人进行了为期 10 个工作日的全面职业暴露评估，测量了颗粒物（PM）的日浓度、金属含量和氧化潜能（OP）。每天收集班前和班后的个人 EBC 和尿液样本。在这些基质中测量了三种氧化应激生物标志物：丙二醛（MDA）、8-羟基-2'脱氧鸟苷（8-OHdG）和 8-异前列腺烷。每种效应生物标志物与暴露变量之间的关联都是通过多变量多层次混合效应模型进行估算的，其中包括滞后时间和非滞后时间：结果：OP 与铁和锰呈正相关，但与任何效应生物标志物均无关联。EBC和尿液中效应生物标志物浓度的变化与可吸入颗粒物中的过渡金属（铜和锌）有关，还与EBC中的特定金属（钡、钴、铬和锰）和尿液中的特定金属（钡、铜、钴、钼、镍、钛和锌）有关。这些关联的方向既与金属有关，也与时间有关。铜或锌与 MDAEBC 之间的关系一般在暴露后 12 或 24 小时后达到统计学意义。EBC和尿液中金属浓度的变化与当天的MDA和8-OHdG浓度相关：EBC和尿液中的MDA与含锌地铁微粒和含铜地铁微粒的反应相反。在 8-OHdG 和尿液中这两种金属的浓度中也观察到了这种锌和铜的分流模式。总的来说，MDA 和 8-OHdG 的反应对两种基质中的当天金属暴露都很敏感。我们建议在大型实地研究中使用 MDA 和 8-OHdG，以解释吸入颗粒物中的金属所引起的氧化应激。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Archives of Occupational and Environmental Health 医学-公共卫生、环境卫生与职业卫生

CiteScore

5.60

自引率

6.70%

发文量

127

审稿时长

3 months

期刊介绍： International Archives of Occupational and Environmental Health publishes Editorials, Review Articles, Original Articles, and Letters to the Editor. It welcomes any manuscripts dealing with occupational or ambient environmental problems, with a special interest in research at the interface of occupational health and clinical medicine. The scope ranges from Biological Monitoring to Dermatology, from Fibers and Dust to Human Toxicology, from Nanomaterials and Ultra-fine Dust to Night- and Shift Work, from Psycho-mental Distress and Burnout to Vibrations. A complete list of topics can be found on the right-hand side under For authors and editors. In addition, all papers should be based on present-day standards and relate to: -Clinical and epidemiological studies on morbidity and mortality -Clinical epidemiological studies on the parameters relevant to the estimation of health risks -Human experimental studies on environmental health effects. Animal experiments are only acceptable if relevant to pathogenic aspects. -Methods for studying the topics mentioned above.