Combined DNA Methylation and Gastric Microbiome Marker Predicts Helicobacter pylori-Negative Gastric Cancer.

IF 3.4 3区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY

Gut and Liver Pub Date : 2024-07-15 Epub Date: 2024-03-21 DOI:10.5009/gnl230348

Min-Jeong Kim, Han-Na Kim, Jonathan P Jacobs, Hyo-Joon Yang

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引用次数: 0

Abstract

Background/aims: While DNA methylation and gastric microbiome are each associated with gastric cancer (GC), their combined role in predicting GC remains unclear. This study investigated the potential of a combined DNA methylation and gastric microbiome signature to predict Helicobacter pylori-negative GC.

Methods: In this case-control study, we conducted quantitative methylation-specific polymerase chain reaction to measure the methylation levels of DKK3, SFRP1, EMX1, NKX6-1, MIR124-3, and TWIST1 in the gastric mucosa from 75 H. pylori-negative patients, including chronic gastritis (CG), intestinal metaplasia (IM), and GC. A combined analysis of DNA methylation and gastric microbiome, using 16S rRNA gene sequencing, was performed in 30 of 75 patients.

Results: The methylation levels of DKK3, SFRP1, EMX1, MIR124-3, and TWIST1 were significantly higher in patients with GC than in controls (all q<0.05). MIR124-3 and TWIST1 methylation levels were higher in patients with IM than those with CG and also in those with GC than in those with IM (all q<0.05). A higher methylation level of TWIST1 was an independent predictor for H. pylori-negative GC after adjusting for age, sex, and atrophy (odds ratio [OR], 15.15; 95% confidence interval [CI], 1.58 to 145.46; p=0.018). The combination of TWIST1 methylation and GC microbiome index (a microbiome marker) was significantly associated with H. pylori-negative GC after adjusting for age, sex, and atrophy (OR, 50.00; 95% CI, 1.69 to 1,476; p=0.024).

Conclusions: The combination of TWIST1 methylation and GC microbiome index may offer potential as a biomarker for predicting H. pylori-negative GC.

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DNA甲基化和胃微生物组标记物联合预测幽门螺旋杆菌阴性胃癌

背景/目的：虽然DNA甲基化和胃微生物组各自与胃癌（GC）相关，但它们在预测GC中的联合作用仍不清楚。本研究调查了 DNA 甲基化和胃微生物组联合特征预测幽门螺旋杆菌阴性 GC 的潜力：在这项病例对照研究中，我们采用定量甲基化特异性聚合酶链反应测量了 75 例幽门螺杆菌阴性患者（包括慢性胃炎（CG）、肠化生（IM）和 GC）胃黏膜中 DKK3、SFRP1、EMX1、NKX6-1、MIR124-3 和 TWIST1 的甲基化水平。利用 16S rRNA 基因测序对 75 例患者中的 30 例进行了 DNA 甲基化和胃微生物组的联合分析：结果：与对照组相比，GC 患者中 DKK3、SFRP1、EMX1、MIR124-3 和 TWIST1 的甲基化水平明显更高（在调整年龄、性别和萎缩程度后，所有 qTWIST1 是幽门螺杆菌阴性 GC 的独立预测因子（几率比 [OR]，15.15；95% 置信区间 [CI]，1.58 至 145.46；P=0.018）。TWIST1甲基化与GC微生物组指数（一种微生物组标记物）的组合与幽门螺杆菌阴性GC显著相关（OR，50.00；95% CI，1.69至1,476；p=0.024）：TWIST1甲基化与GC微生物组指数的结合可能成为预测幽门螺杆菌阴性GC的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gut and Liver 医学-胃肠肝病学

CiteScore

7.50

自引率

8.80%

发文量

119

审稿时长

6-12 weeks

期刊介绍： Gut and Liver is an international journal of gastroenterology, focusing on the gastrointestinal tract, liver, biliary tree, pancreas, motility, and neurogastroenterology. Gut and Liver delivers up-to-date, authoritative papers on both clinical and research-based topics in gastroenterology. The Journal publishes original articles, case reports, brief communications, letters to the editor and invited review articles in the field of gastroenterology. The Journal is operated by internationally renowned editorial boards and designed to provide a global opportunity to promote academic developments in the field of gastroenterology and hepatology. Gut and Liver is jointly owned and operated by 8 affiliated societies in the field of gastroenterology, namely: the Korean Society of Gastroenterology, the Korean Society of Gastrointestinal Endoscopy, the Korean Society of Neurogastroenterology and Motility, the Korean College of Helicobacter and Upper Gastrointestinal Research, the Korean Association for the Study of Intestinal Diseases, the Korean Association for the Study of the Liver, the Korean Pancreatobiliary Association, and the Korean Society of Gastrointestinal Cancer.