Isolation, Identification, and Characterization of Bacillus cereus Group Bacteria Isolated from the Dairy Farm Environment and Raw Milk in Tunisia.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2024-03-18 DOI:10.1089/fpd.2023.0154

Randa Ben Akacha, Maroua Gdoura-Ben Amor, Hanen Sellami, Noël Grosset, Sophie Jan, Michel Gautier, Radhouane Gdoura

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Abstract

Members of the Bacillus cereus group are well-known opportunistic foodborne pathogens. In this study, the prevalence, hemolytic activity, antimicrobial resistance profile, virulence factor genes, genetic diversity by enterobacterial repetitive intergenic consensus (ERIC)-polymerase chain reaction (PCR) genotyping, and adhesion potential were investigated in isolates from a Tunisian dairy farm environment and raw milk. A total of 200 samples, including bedding, feces, feed, liquid manure, and raw bovine milk, were examined. Based on PCR test targeting sspE gene, 59 isolates were detected. The prevalence of B. cereus group isolates in bedding, feces, liquid manure, feed, and raw milk was 48%, 37.8%, 20%, 17.1%, and 12.5%, respectively. Out of the tested strains, 81.4% showed β-hemolytic on blood agar plates. An antimicrobial resistance test against 11 antibiotics showed that more than 50% of the isolates were resistant to ampicillin and novobiocin, while a high sensitivity to other antibiotics tested was observed in most isolates. The distribution of enterotoxigenic genes showed that 8.5% and 67.8% of isolates carried hblABCD and nheABC, respectively. In addition, the detection rate of cytotoxin K (cytk), enterotoxin T (bceT), and ces genes was 72.9%, 64.4%, and 5.1%, respectively. ERIC-PCR fingerprinting genotype analysis allowed discriminating 40 different profiles. The adhesion potential of B. cereus group on stainless steel showed that all isolates were able to adhere at various levels, from 1.5 ± 0.3 to 5.1 ± 0.1 log colony-forming unit (CFU)/cm² for vegetative cells and from 2.6 ± 0.4 to 5.7 ± 0.3 log CFU/cm² for spores. An important finding of the study is useful for updating the knowledge of the contamination status of B. cereus group in Tunisia, at the dairy farm level.

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从突尼斯奶牛场环境和生奶中分离出的蜡样芽孢杆菌属细菌的分离、鉴定和特征描述。

蜡样芽孢杆菌属成员是众所周知的机会性食源性致病菌。本研究通过肠杆菌重复基因间共识（ERIC）-聚合酶链式反应（PCR）基因分型，调查了突尼斯奶牛场环境和生奶中分离物的流行率、溶血活性、抗菌药耐药性特征、毒力因子基因、遗传多样性和粘附潜力。共检测了 200 份样本，包括垫料、粪便、饲料、液体粪便和生牛乳。根据针对 sspE 基因的 PCR 检测，共检测到 59 个分离株。在垫料、粪便、液体粪便、饲料和生牛奶中，蜡样芽孢杆菌群分离株的流行率分别为 48%、37.8%、20%、17.1% 和 12.5%。在检测的菌株中，81.4%在血琼脂平板上显示出β-溶血性。对 11 种抗生素的耐药性测试表明，50% 以上的分离菌株对氨苄西林和新生物菌素具有耐药性，而大多数分离菌株对其他测试抗生素具有高度敏感性。肠毒性基因的分布显示，分别有 8.5% 和 67.8% 的分离物携带 hblABCD 和 nheABC。此外，细胞毒素 K（cytk）、肠毒素 T（bceT）和 ces 基因的检出率分别为 72.9%、64.4% 和 5.1%。ERIC-PCR 指纹基因型分析可区分 40 种不同的基因型。蜡样芽孢杆菌群在不锈钢上的附着潜力表明，所有分离物都能在不同水平上附着，无性细胞为 1.5 ± 0.3 至 5.1 ± 0.1 对数菌落形成单位（CFU）/cm2，孢子为 2.6 ± 0.4 至 5.7 ± 0.3 对数菌落形成单位/cm2。该研究的一项重要发现有助于更新突尼斯奶牛场对蜡样芽孢杆菌污染状况的认识。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.