T. I. Nikolaeva, D. A. Barsuk, M. V. Molchanov, D. A. Prokhorov, V. I. Emelyanenko, P. V. Shekhovtsov
{"title":"Comparative Analysis of the Degree of Hydrolysis of Biopolymers in Hyaline Cartilage Homogenates in the Presence of Proteolytic Enzymes","authors":"T. I. Nikolaeva, D. A. Barsuk, M. V. Molchanov, D. A. Prokhorov, V. I. Emelyanenko, P. V. Shekhovtsov","doi":"10.1134/s0006350923060143","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The study of the degree of hydrolysis depending on the action of various proteolytic enzymes is one of the tasks in the development of nutraceuticals from connective tissues for biomedicine. Hydrolysis of biopolymers in hyaline cartilage homogenates from the trachea of cattle and pigs was carried out under the action of the enzymes pancreatin, chymopsin, papain, and the proteolytic drug karipazim containing papain. It has been shown that karipazim produced by MedFlorina acted more effectively than karipazim produced by Vifitech, and the degree of collagen hydrolysis was maximal at 60°C and a concentration of karipazim of 10%. A more complete hydrolysis of proteoglycans was observed in the homogenates of hyaline cartilage of cattle, since glucose, the final product of glycosaminoglycan hydrolysis, was identified on NMR spectra.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":null,"pages":null},"PeriodicalIF":4.0330,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysics","FirstCategoryId":"4","ListUrlMain":"https://doi.org/10.1134/s0006350923060143","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
The study of the degree of hydrolysis depending on the action of various proteolytic enzymes is one of the tasks in the development of nutraceuticals from connective tissues for biomedicine. Hydrolysis of biopolymers in hyaline cartilage homogenates from the trachea of cattle and pigs was carried out under the action of the enzymes pancreatin, chymopsin, papain, and the proteolytic drug karipazim containing papain. It has been shown that karipazim produced by MedFlorina acted more effectively than karipazim produced by Vifitech, and the degree of collagen hydrolysis was maximal at 60°C and a concentration of karipazim of 10%. A more complete hydrolysis of proteoglycans was observed in the homogenates of hyaline cartilage of cattle, since glucose, the final product of glycosaminoglycan hydrolysis, was identified on NMR spectra.
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍：
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
