Reliable quality of R-phycoerythrin derived from Portieria hornemannii for effective antioxidant, antibacterial, and anticancer activity

Biomedical engineering advances Pub Date : 2024-02-21 DOI:10.1016/j.bea.2024.100116

Saranya Karuppannan , Mani Sivakumar , Balasubramani Govindasamy , Santhosh Chinnaraj , Viji Maluventhan , Maruthupandian Arumugam

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Abstract

The phycobiliprotein is a light-harvesting fluorescent probe; Phycoerythrin is a pigment used in many industrial, and pharmaceutical applications; including fluorescence resonance energy transfer and flow cytometry. The main objective of the current study was purifying the phycoerythrin in Portieria hornemannii. The phycoerythrin pigment was dialyzed commonly using Q sepharose, but it is high cost. However, the alternative method is DEAE-Sephadex is cost-effective, high- yield, pure compound, and has high activity performance. Then the R-Phycoerythrin (R-PE) compound was extracted in 0.02 M of phosphate buffer (pH–7.2). R-PE investigated the FT-IR, HPLC, SDS-PAGE and including physico-chemical properties. Then the purified R-PE compound was evaluated by an antibacterial, antioxidant, and anticancer activity. The R-Phycoerythrin was showing a maximum inhibition effect of high concentration (IC₅₀value 77.98 µg/ml) against the colon cancer HT-29 cell line.

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质量可靠的 R-phycoerythrin 提取自 Portieria hornemannii，具有有效的抗氧化、抗菌和抗癌活性

藻胆蛋白是一种光收集荧光探针；藻红蛋白是一种色素，可用于多种工业和医药应用，包括荧光共振能量转移和流式细胞术。本研究的主要目的是纯化 Portieria hornemannii 中的藻红蛋白。通常使用 Q sepharose 来透析藻红素色素，但这种方法成本较高。然而，替代方法 DEAE-Sephadex 具有成本低、产率高、化合物纯度高和活性高等优点。然后，在 0.02 M 磷酸盐缓冲液（pH-7.2）中提取 R-聚乙二醇（R-PE）化合物。对 R-PE 进行了傅立叶变换红外光谱（FT-IR）、高效液相色谱（HPLC）、SDS-PAGE 等物理化学性质的研究。然后对纯化的 R-PE 复合物进行了抗菌、抗氧化和抗癌活性评估。R-Phycoerythrin 对结肠癌 HT-29 细胞株具有高浓度（IC50 值为 77.98 µg/ml）的最大抑制作用。

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Biomedical engineering advances Bioengineering, Biomedical Engineering

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59 days