Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D

IF 12.2 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Current Opinion in Solid State & Materials Science Pub Date : 2024-03-18 DOI:10.1016/j.cossms.2024.101147

Jongchan Park, Liang Gao

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Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool offering molecular specific insights into samples through the measurement of fluorescence decay time, with promising applications in diverse research fields. However, to acquire two-dimensional lifetime images, conventional FLIM relies on extensive scanning in both the spatial and temporal domain, resulting in much slower acquisition rates compared to intensity-based approaches. This problem is further magnified in three-dimensional imaging, as it necessitates additional scanning along the depth axis. Recent advancements have aimed to enhance the speed and three-dimensional imaging capabilities of FLIM. This review explores the progress made in addressing these challenges and discusses potential directions for future developments in FLIM instrumentation.

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荧光寿命成像显微镜仪器的进步：实现高速和三维

荧光寿命成像显微镜（FLIM）是一种功能强大的成像工具，可通过测量荧光衰减时间深入了解样品的分子特异性，在多个研究领域有着广阔的应用前景。然而，要获取二维荧光寿命图像，传统的 FLIM 需要在空间域和时间域进行大量扫描，与基于强度的方法相比，获取速度要慢得多。在三维成像中，这一问题被进一步放大，因为它需要沿深度轴进行额外的扫描。最近的进步旨在提高 FLIM 的速度和三维成像能力。本综述探讨了在应对这些挑战方面取得的进展，并讨论了 FLIM 仪器未来发展的潜在方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Opinion in Solid State & Materials Science 工程技术-材料科学：综合

CiteScore

21.10

自引率

3.60%

发文量

审稿时长

47 days

期刊介绍： Title: Current Opinion in Solid State & Materials Science Journal Overview: Aims to provide a snapshot of the latest research and advances in materials science Publishes six issues per year, each containing reviews covering exciting and developing areas of materials science Each issue comprises 2-3 sections of reviews commissioned by international researchers who are experts in their fields Provides materials scientists with the opportunity to stay informed about current developments in their own and related areas of research Promotes cross-fertilization of ideas across an increasingly interdisciplinary field