Temozolomide nano-in-nanofiber delivery system with sustained release and enhanced cellular uptake by U87MG cells.

IF 2.4 4区医学 Q3 CHEMISTRY, MEDICINAL

Drug Development and Industrial Pharmacy Pub Date : 2024-05-01 Epub Date: 2024-04-01 DOI:10.1080/03639045.2024.2332906

Karishma Shetty, Khushwant S Yadav

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引用次数: 0

Abstract

Objective: The study was aimed at formulating temozolomide (TMZ) loaded gelatin nanoparticles (GNPs) encapsulated into polyvinyl alcohol (PVA) nanofibers (TMZ-GNPs-PVA NFs) as the nano-in-nanofiber delivery system. The secondary objective was to explore the sustained releasing ability of this system and to assess its enhanced cellular uptake against U87MG glioma cells in vitro.

Significance: Nano-in-nanofibers are the emerging drug delivery systems for treating a wide range of diseases including cancers as they overcome the challenges experienced by nanoparticles and nanofibers alone.

Methods: The drug-loaded GNPs were formulated by one-step desolvation method. The Design of Experiments (DoE) was used to optimize nanoparticle size and entrapment efficiency. The optimized drug-loaded nanoparticles were then encapsulated within nanofibers using blend electrospinning technique. The U87MG glioma cells were used to investigate the uptake of the formulation.

Results: A 3² factorial design was used to optimize the mean particle size (145.7 nm) and entrapment efficiency (87.6%) of the TMZ-loaded GNPs which were subsequently ingrained into PVA nanofibers by electrospinning technique. The delivery system achieved a sustained drug release for up to seven days (in vitro). The SEM results ensured that the expected nano-in-nanofiber delivery system was achieved. The uptake of TMZ-GNPs-PVA NFs by cells was increased by a factor of 1.964 compared to that of the pure drug.

Conclusion: The nano-in-nanofiber drug delivery system is a potentially useful therapeutic strategy for the management of glioblastoma multiforme.

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具有持续释放和增强 U87MG 细胞摄取能力的替莫唑胺纳米纤维给药系统。

研究目的该研究旨在将负载明胶纳米颗粒（GNPs）的替莫唑胺（TMZ）封装到聚乙烯醇（PVA）纳米纤维（TMZ-GNPs-PVA NFs）中，作为纳米纤维中的纳米给药系统。次要目的是探索该系统的持续释放能力，并在体外评估其对 U87MG 胶质瘤细胞的增强细胞摄取能力：纳米中纳米纤维是治疗包括癌症在内的多种疾病的新兴药物递送系统，因为它们克服了纳米颗粒和纳米纤维单独使用所面临的挑战：方法：采用一步脱溶法制备了载药明胶纳米颗粒。方法：采用一步脱溶法配制了载药明胶纳米粒子，并利用实验设计法（DoE）优化了纳米粒子的尺寸和夹持效率。然后利用混合电纺技术将优化后的载药纳米粒子封装在纳米纤维中。结果：结果：采用32因子设计优化了TMZ负载明胶纳米颗粒（GNPs）的平均粒径（145.7nm）和夹带效率（87.6%），随后通过电纺丝技术将GNPs植入PVA纳米纤维中。该给药系统实现了长达 7 天的持续药物释放（体外）。扫描电子显微镜结果表明，纳米纤维中的纳米给药系统达到了预期的效果。与纯药物相比，细胞对 TMZ-GNPs-PVA NFs 的吸收增加了 1.964 倍：纳米纤维内给药系统是治疗多形性胶质母细胞瘤的一种潜在有效的治疗策略。

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来源期刊

Drug Development and Industrial Pharmacy 医学-药学

CiteScore

6.80

自引率

0.00%

发文量

审稿时长

4.5 months

期刊介绍： The aim of Drug Development and Industrial Pharmacy is to publish novel, original, peer-reviewed research manuscripts within relevant topics and research methods related to pharmaceutical research and development, and industrial pharmacy. Research papers must be hypothesis driven and emphasize innovative breakthrough topics in pharmaceutics and drug delivery. The journal will also consider timely critical review papers.