Synthesis of nonembryonic synseed, short term conservation, phytochemical evaluation and assessment of genetic stability through SCoT markers in Decalepis salicifolia
IF 2.3 3区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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引用次数: 0
Abstract
The current work specifies an effort to conserve the highly endangered plant Decalepis salicifolia by utilizing encapsulation technology for high plantlet recovery, short-term storage, and conservation. In order to determine the best culture conditions for maximizing the ability of synseeds to develop into complete plantlets, nodal segments (NS) were encapsulated in a sodium alginate (SA) matrix. The best solution for creating isodiametric beads was determined to be a 3% sodium alginate with 100 mM CaCl2 solution. Regeneration of the encapsulated segment was reported directly or after storage at 4 °C for up to 8 weeks. The highest shoot regrowth frequency (90.8%) and highest microshoots per encapsulated nodal segment i.e. 4.90 were recorded when Murashige and Skoog (MS) basal medium was supplemented with 5.0 µM 6-benzyl adenine (BA) + 1.0 µM indole-3-butyric acid (IBA) and encapsulated nodal segment were inoculated onto the nutrient medium comprised of MS + 5.0 µM BA. Microshoots rooted effectively on MS medium augmented with indole-3-butyric acid IBA (2.5 µM). Plantlets achieved from preserved synthetic seeds were acclimatized and relocated in the natural condition successfully with an immortality rate of 87.1%. Subsequently, it was planted in garden soil and exhibited no morphological changes. Gas Chromatography and Mass Spectrometry (GC-MS) of leaf extract, obtained from the donor plant as well as in vitro derived encapsulated regenerated plantlets shows the presence of diverse chemical compounds of immense pharmacological properties and number of biochemical compounds are almost similar in both, which established the biochemical similarity between them. Genetic similarity between the donar plant and the synseed-derived plant was confirmed by the presence of monomorphic bands produced with the help of the start codon targeted (SCoT) marker.
期刊介绍：
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
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