Differentiation of ovarian serous carcinoma from ovarian clear cell carcinoma using a 10-gene signature selected by comprehensive gene expression analysis.

IF 0.7 Q3 MEDICINE, GENERAL & INTERNAL

Fukushima Journal of Medical Science Pub Date : 2024-04-26 Epub Date: 2024-03-15 DOI:10.5387/fms.23-00011

Shinji Nomura, Takafumi Watanabe, Reiko Honma, Susumu Matsukura, Emi Ito, Jun-Ichi Imai, Yuichiro Kiko, Osamu Suzuki, Yuko Hashimoto, Manabu Kojima, Shigenori Furukawa, Shu Soeda, Shinya Watanabe, Keiya Fujimori

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引用次数: 0

Abstract

Aim: Ovarian serous carcinoma (OSC) and ovarian clear cell carcinoma (OCCC) are two major histological types of epithelial ovarian carcinoma (EOC), each with different biological features and clinical behaviors. Although immunostaining is commonly used for differential diagnosis between OSC and OCCC, correct identification of EOC with mixed-type histology is sometimes a diagnostic challenge. The aim of the present study was to explore candidate genes as potential diagnostic biomarkers that distinguish OSC from OCCC.

Methods: A total of 57 surgical specimens were obtained from EOC patients who had previously undergone primary debulking surgery. Total RNAs were extracted from fresh-frozen tissues of EOC patients, and were used for comprehensive gene expression analysis using DNA microarray technology.

Results: Ten candidate genes, FXYD2, TMEM101, GABARAPL1, ARG2, GLRX, RBPMS, GDF15, PPP1R3B, TOB1, and GSTM3 were up-regulated in OCCC compared to OSC. All EOC patients were divided into two groups according to hierarchical clustering using a 10-gene signature.

Conclusion: Our data suggest that the 10 candidate genes would be an excellent marker for distinguishing OSC from OCCC. Furthermore, the molecular signatures of the 10 genes may enlighten us on the differences in carcinogenesis, and provide a theoretical basis for OCCC's resistance to chemotherapy in the future.

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利用综合基因表达分析选出的 10 个基因特征区分卵巢浆液性癌和卵巢透明细胞癌。

目的：卵巢浆液性癌（OSC）和卵巢透明细胞癌（OCCC）是上皮性卵巢癌（EOC）的两种主要组织学类型，各自具有不同的生物学特征和临床表现。尽管免疫染色通常用于鉴别诊断卵巢上皮细胞癌和卵巢透明细胞癌，但正确识别混合型组织学的 EOC 有时仍是一项诊断难题。本研究旨在探索候选基因作为潜在的诊断生物标志物，以区分OSC和OCCC：方法：本研究共从 57 例 EOC 患者身上获取了手术标本，这些患者都曾接受过初级清扫手术。从 EOC 患者的新鲜冷冻组织中提取总 RNA，并利用 DNA 微阵列技术进行全面的基因表达分析：结果：与 OSC 相比，10 个候选基因 FXYD2、TMEM101、GABARAPL1、ARG2、GLRX、RBPMS、GDF15、PPP1R3B、TOB1 和 GSTM3 在 OCCC 中上调。结论：我们的数据表明，10个候选基因在OCCC和OSC中被上调：我们的数据表明，10个候选基因是区分OSC和OCCC的绝佳标志。结论：我们的数据表明，这 10 个候选基因将是区分 OSC 和 OCCC 的绝佳标志物。此外，这 10 个基因的分子特征可能有助于我们了解癌变过程中的差异，并为将来 OCCC 对化疗的耐药性提供理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fukushima Journal of Medical Science MEDICINE, GENERAL & INTERNAL-

CiteScore

1.70

自引率

12.50%

发文量