[BMP9 promotes osteogenic differentiation of periodontal ligament stem cells under inflammatory environment via ERK5/KLF4 signaling pathway].

Q4 Medicine

上海口腔医学 Pub Date : 2023-12-01

Li Li, Wen-Wei Lian

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引用次数: 0

Abstract

Purpose: To explore how bone morphogenetic protein 9(BMP9) promotes the osteogenic differentiation of periodontal ligament stem cells(PDLSCs) via ERK5/KLF4 signaling pathway under an inflammatory environment.

Methods: Recombinant adenovirus to overexpress BMP9 in PDLSCs stimulated with TNF-α was used and the expression level of osteogenic-related genes and proteins in BMP9-treated cells was examined using RT-PCR and Western blot. Then, KLF4 was overexpressed in PDLSCs via transfection and subjected to inflammatory stimulation to observe its effects on osteogenic differentiation and the expression of osteogenic-related genes. overexpression of BMP9 under the condition of KLF4 knockdown was conducted and its effects on osteogenic differentiation and the expression of osteogenic-related genes was analyzed. ERK5 inhibitor BIX02189 was used to explore the role of ERK5 in BMP9-induced osteogenic differentiation. Statistical analysis was performed with SPSS 20.0 software package.

Results: BMP9 could promote the osteogenic differentiation of PDLSCs and the expression of KLF4 in an inflammatory environment. Moreover, the overexpression of KLF4 further promoted the osteogenic differentiation of PDLSCs. However, when KLF4 was knocked down, the osteogenic differentiation promotion effect of BMP9 on PDLSCs was weakened. After BMP9 treatment, the phosphorylation level of ERK5 significantly increased. However, after adding ERK5 inhibitor, the promoting effect of BMP9 on osteogenic differentiation was significantly reduced.

Conclusions: BMP9 can promote the osteogenic differentiation of PDLSCs via ERK5/KLF4 signaling pathway in an inflammatory environment.

本刊更多论文

[BMP9通过ERK5/KLF4信号通路促进炎症环境下牙周韧带干细胞的成骨分化】。］

目的：探讨在炎症环境下骨形态发生蛋白9（BMP9）如何通过ERK5/KLF4信号通路促进牙周韧带干细胞（PDLSCs）的成骨分化：方法：利用重组腺病毒在TNF-α刺激下的PDLSCs中过表达BMP9，并用RT-PCR和Western blot检测BMP9处理细胞中成骨相关基因和蛋白的表达水平。然后，通过转染在 PDLSCs 中过表达 KLF4，并对其进行炎症刺激，观察其对成骨分化和成骨相关基因表达的影响；在敲除 KLF4 的条件下过表达 BMP9，分析其对成骨分化和成骨相关基因表达的影响。使用 ERK5 抑制剂 BIX02189 探讨 ERK5 在 BMP9 诱导的成骨分化中的作用。统计分析采用SPSS 20.0软件包：结果：BMP9能促进炎症环境中PDLSCs的成骨分化和KLF4的表达。此外，KLF4的过表达进一步促进了PDLSCs的成骨分化。然而，当KLF4被敲除后，BMP9对PDLSCs成骨分化的促进作用减弱。BMP9处理后，ERK5的磷酸化水平明显升高。结论：BMP9能促进PDLSCs的成骨分化：结论：在炎症环境中，BMP9可通过ERK5/KLF4信号通路促进PDLSCs的成骨分化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

上海口腔医学 Medicine-Medicine (all)

CiteScore

0.30

自引率

0.00%

发文量

5299

期刊介绍： "Shanghai Journal of Stomatology (SJS)" is a comprehensive academic journal of stomatology directed by Shanghai Jiao Tong University and sponsored by the Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. The main columns include basic research, clinical research, column articles, clinical summaries, reviews, academic lectures, etc., which are suitable for reference by clinicians, scientific researchers and teaching personnel at all levels engaged in oral medicine.