Diagnostic Value of GSTP1, RASSF1, AND RASSF2 Methylation in Serum of Prostate Cancer Patients.

IF 1.5 4区医学 Q3 UROLOGY & NEPHROLOGY

Urology Journal Pub Date : 2024-05-06 DOI:10.22037/uj.v20i.8014

Emre Aykanli, Serdar Arisan, Elif Damla Arisan, Abdullah Hizir Yavuzsan

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引用次数: 0

Abstract

Purpose: Considering the inadequacy of PSA measurement in the diagnosis of prostate cancer, it is aimed to establish a potential liquid biopsy diagnostic panel.

Materials and methods: 39 patients who underwent TRUS-biopsy and 15 healthy volunteers were included. Approximately 15 ml of venous blood samples taken from healthy volunteers and patients before biopsy were separated as plasma. Hypermethylation status of GSTP1 and RASSF1:RASSF2 genes was revealed in cfDNA materials collected from plasma samples. Correlation of this epigenetic change detected in PCa, BPH and healthy volunteer groups with pathology results was examined.

Results: Pathology reports of 39 patients included were 13 PCa, 3 ASAP, 3 HGPIN, and 20 BPH. In total, 3 of the patients with PCa had positive GSTP1, 4 had RASSF1 and 9 had positive RASSF2 methylation. It was seen that RASSF2 had the highest sensitivity (69%), specificity (39%) and NPV (80%), while RASSF1 had the highest PPV (30%). When the binary combinations of genes were examined it was observed that the GSTP1:RASSF1 combination had the highest sensitivity (46%), specificity (76%) and NPV (82%). When the methylation of all three genes was examined, it was observed that the sensitivity was quite low (8%), but the specificity (83%) increased significantly.

Conclusion: Although we observed that the GSTP1 and RASSF1 methylation positivity rates that we examined in our study were higher in patients without prostate cancer, we found that the RASSF2 methylation rate was higher in patients with prostate cancer. randomized controlled studies are needed.

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前列腺癌患者血清中 GSTP1、RASSF1 和 RASSF2 甲基化的诊断价值

目的：考虑到 PSA 测量在前列腺癌诊断中的不足，我们旨在建立一个潜在的液体活检诊断面板。材料：纳入 39 名接受 TRUS 活检的患者和 15 名健康志愿者，活检前从健康志愿者和患者身上采集约 15 毫升静脉血样本，分离为血浆。从血浆样本中收集的 cfDNA 材料显示了 GSTP1 和 RASSF1:RASSF2 基因的高甲基化状态。研究了在 PCa、良性前列腺增生症和健康志愿者组中检测到的这种表观遗传学变化与病理学结果的相关性：39 名患者的病理报告包括 13 例 PCa、3 例 ASAP、3 例 HGPIN 和 20 例良性前列腺增生。其中，3 名 PCa 患者的 GSTP1 呈阳性，4 名患者的 RASSF1 呈阳性，9 名患者的 RASSF2 甲基化呈阳性。结果显示，RASSF2 的灵敏度（69%）、特异性（39%）和 NPV（80%）最高，而 RASSF1 的 PPV（30%）最高。在检测基因的二元组合时，发现 GSTP1:RASSF1 组合的灵敏度（46%）、特异性（76%）和 NPV（82%）最高。当检测所有三个基因的甲基化时，发现灵敏度相当低（8%），但特异性（83%）显著增加：结论：尽管我们在研究中观察到 GSTP1 和 RASSF1 甲基化阳性率在非前列腺癌患者中更高，但我们发现 RASSF2 甲基化率在前列腺癌患者中更高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Urology Journal UROLOGY & NEPHROLOGY-

CiteScore

2.60

自引率

6.70%

发文量

审稿时长

6-12 weeks

期刊介绍： As the official journal of the Urology and Nephrology Research Center (UNRC) and the Iranian Urological Association (IUA), Urology Journal is a comprehensive digest of useful information on modern urology. Emphasis is on practical information that reflects the latest diagnostic and treatment techniques. Our objectives are to provide an exceptional source of current and clinically relevant research in the discipline of urology, to reflect the scientific work and progress of our colleagues, and to present the articles in a logical, timely, and concise format that meets the diverse needs of today’s urologist. Urology Journal publishes manuscripts on urology and kidney transplantation, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. Accordingly, original articles, case reports, and letters to editor are encouraged.