Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2024-06-01 Epub Date: 2024-03-16 DOI:10.1007/s10529-024-03472-5
Qiang Wilson Yan, Ying-Chun Liu, Christa Barrett, Kelly Haake, Daniel Seeler, Oliver May, Ross Zirkle
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引用次数: 0

Abstract

Objective: Currently, there is lack of a consistent and highly enriched source for docosapentaenoic acid (n-3 DPA, C22:5), and this work report the isolation of microorganism that naturally produces n-3 DPA.

Results: In this work, we screened microorganisms in our culture collections with the goal to isolate a strain with high levels of n-3 DPA. We isolated a strain of Sphaeroforma arctica that produces up to 11% n-3 DPA in total fatty acid and has a high n-3 DPA to DHA/EPA ratio. The cell growth of the isolated strain was characterized using microscopy imaging and flow cytometer technologies to confirm the coenocytic pattern of cell divisions previously described in S. arctica. Our novel isolate of S. arctica grew more robustly and produced significantly more n-3 DPA compared to previously isolated and described strains indicating the uniqueness of the discovered strain.

Conclusion: Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.

Abstract Image

在海洋鱼类 Sphaeroforma arctica 的新分离物中积累二十二碳五烯酸(n-3 DPA)。
目的:目前,二十二碳五烯酸(n-3 DPA,C22:5)缺乏一致且高度富集的来源,这项工作报告了天然产生 n-3 DPA 的微生物的分离情况:在这项工作中,我们筛选了培养物中的微生物,目的是分离出一株含有大量 n-3 DPA 的菌株。我们分离出了一株 Sphaeroforma arctica 菌株,该菌株产生的 n-3 DPA 占总脂肪酸的 11%,并且 n-3 DPA 与 DHA/EPA 的比率很高。我们利用显微镜成像和流式细胞仪技术对分离菌株的细胞生长进行了鉴定,证实了之前在北极鞘氨醇中描述的细胞分裂的同源模式。与之前分离和描述的菌株相比,我们新分离出的北极鼠的生长更健壮,产生的 n-3 DPA 明显更多,这表明了所发现菌株的独特性:总之,这项工作报告了第一个分离的 n-3 DPA 生产微生物,并为未来菌株改良和阐明这种低碳-PUFA 对人类营养和健康的生理功能奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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