Isothermal titration calorimetry and surface plasmon resonance methods to probe protein–protein interactions

IF 4.2 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Methods Pub Date : 2024-03-15 DOI:10.1016/j.ymeth.2024.03.007

Vaibhav Upadhyay , Alexandra Lucas , Casey Patrick, Krishna M.G. Mallela

引用次数: 0

Abstract

Isothermal titration calorimetry (ITC) and surface plasmon resonance (SPR) are two commonly used methods to probe biomolecular interactions. ITC can provide information about the binding affinity, stoichiometry, changes in Gibbs free energy, enthalpy, entropy, and heat capacity upon binding. SPR can provide information about the association and dissociation kinetics, binding affinity, and stoichiometry. Both methods can determine the nature of protein–protein interactions and help understand the physicochemical principles underlying complex biochemical pathways and communication networks. This methods article discusses the practical knowledge of how to set up and troubleshoot these two experiments with some examples.

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等温滴定量热法和表面等离子体共振法探测蛋白质与蛋白质之间的相互作用。

等温滴定量热法（ITC）和表面等离子体共振（SPR）是探测生物分子相互作用的两种常用方法。等温滴定法可提供有关结合亲和力、化学计量学、结合时吉布斯自由能、焓、熵和热容量变化的信息。SPR 可提供有关结合和解离动力学、结合亲和力和化学计量学的信息。这两种方法都能确定蛋白质-蛋白质相互作用的性质，有助于理解复杂的生化途径和通讯网络所依据的物理化学原理。本方法文章通过一些实例讨论了如何设置这两种实验并排除故障的实用知识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods 生物-生化研究方法

CiteScore

9.80

自引率

2.10%

发文量

222

审稿时长

11.3 weeks

期刊介绍： Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.