Preparation of Short Collagen Nanofibers for Injectable Hydrogels: Comparative Assessment of Fragmentation Methods, Physicomechanical Properties, and Biocompatibility
IF 4.2 3区 材料科学Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY
{"title":"Preparation of Short Collagen Nanofibers for Injectable Hydrogels: Comparative Assessment of Fragmentation Methods, Physicomechanical Properties, and Biocompatibility","authors":"Ayoob Karimizade, Amir Mellati","doi":"10.1002/mame.202300460","DOIUrl":null,"url":null,"abstract":"<p>Collagen nanofibers can be employed in hydrogels to create injectable nanocomposite hydrogels, mimicking the fibrous architecture of the natural extracellular matrix (ECM). As long continuous electrospun collagen nanofibers are not applicable, fragmentation is inevitable to obtain injectable hydrogels with a fine viscosity. Here, four methods: hand grinding (HG), homogenizer (HM), mixer milling (MM), and ultrasonication (UH) are used to disintegrate and shorten collagen nanofiber mats before incorporation into an injectable hyaluronic acid hydrogel as a matrix. The Length-to-diameter (<i>L</i>/<i>d</i>) ratio and morphology of fragmented collagen are compared by SEM. The injection force, mechanical properties, and cell viability of the selected collagen-incorporated hydrogels are also evaluated. UH emerges as the most effective method, yielding the highest <i>L</i>/<i>d</i> ratio of 46 and a notable compressive modulus of 8.7 ± 0.92 kPa. Assessment of the in vitro cell viability of the encapsulated chondrocytes in the collagen-incorporated hydrogels demonstrates good biocompatibility, and hydrogels containing UH short nanofiber, in particular, show an increase in cell proliferation. This work indicates how collagen mats can be effectively broken down and combined with injectable hydrogels to enhance both their mechanical behavior and biocompatibility.</p>","PeriodicalId":18151,"journal":{"name":"Macromolecular Materials and Engineering","volume":"309 6","pages":""},"PeriodicalIF":4.2000,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mame.202300460","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Macromolecular Materials and Engineering","FirstCategoryId":"88","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mame.202300460","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Collagen nanofibers can be employed in hydrogels to create injectable nanocomposite hydrogels, mimicking the fibrous architecture of the natural extracellular matrix (ECM). As long continuous electrospun collagen nanofibers are not applicable, fragmentation is inevitable to obtain injectable hydrogels with a fine viscosity. Here, four methods: hand grinding (HG), homogenizer (HM), mixer milling (MM), and ultrasonication (UH) are used to disintegrate and shorten collagen nanofiber mats before incorporation into an injectable hyaluronic acid hydrogel as a matrix. The Length-to-diameter (L/d) ratio and morphology of fragmented collagen are compared by SEM. The injection force, mechanical properties, and cell viability of the selected collagen-incorporated hydrogels are also evaluated. UH emerges as the most effective method, yielding the highest L/d ratio of 46 and a notable compressive modulus of 8.7 ± 0.92 kPa. Assessment of the in vitro cell viability of the encapsulated chondrocytes in the collagen-incorporated hydrogels demonstrates good biocompatibility, and hydrogels containing UH short nanofiber, in particular, show an increase in cell proliferation. This work indicates how collagen mats can be effectively broken down and combined with injectable hydrogels to enhance both their mechanical behavior and biocompatibility.
期刊介绍:
Macromolecular Materials and Engineering is the high-quality polymer science journal dedicated to the design, modification, characterization, processing and application of advanced polymeric materials, including membranes, sensors, sustainability, composites, fibers, foams, 3D printing, actuators as well as energy and electronic applications.
Macromolecular Materials and Engineering is among the top journals publishing original research in polymer science.
The journal presents strictly peer-reviewed Research Articles, Reviews, Perspectives and Comments.
ISSN: 1438-7492 (print). 1439-2054 (online).
Readership:Polymer scientists, chemists, physicists, materials scientists, engineers
Abstracting and Indexing Information:
CAS: Chemical Abstracts Service (ACS)
CCR Database (Clarivate Analytics)
Chemical Abstracts Service/SciFinder (ACS)
Chemistry Server Reaction Center (Clarivate Analytics)
ChemWeb (ChemIndustry.com)
Chimica Database (Elsevier)
COMPENDEX (Elsevier)
Current Contents: Physical, Chemical & Earth Sciences (Clarivate Analytics)
Directory of Open Access Journals (DOAJ)
INSPEC (IET)
Journal Citation Reports/Science Edition (Clarivate Analytics)
Materials Science & Engineering Database (ProQuest)
PASCAL Database (INIST/CNRS)
Polymer Library (iSmithers RAPRA)
Reaction Citation Index (Clarivate Analytics)
Science Citation Index (Clarivate Analytics)
Science Citation Index Expanded (Clarivate Analytics)
SciTech Premium Collection (ProQuest)
SCOPUS (Elsevier)
Technology Collection (ProQuest)
Web of Science (Clarivate Analytics)