Exploring the role of GhN/AINV23: implications for plant growth, development, and drought tolerance

IF 5.7 2区生物学 Q1 BIOLOGY

Biology Direct Pub Date : 2024-03-14 DOI:10.1186/s13062-024-00465-2

Kaikai Qiao, Qingtao Zeng, Jiaoyan Lv, Lingling Chen, Juxin Hao, Ding Wang, Qifeng Ma, Shuli Fan

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引用次数: 0

Abstract

Neutral/alkaline invertases (N/AINVs) play a crucial role in plant growth, development, and stress response, by irreversibly hydrolyzing sucrose into glucose and fructose. However, research on cotton in this area is limited. This study aims to investigate GhN/AINV23, a neutral/alkaline invertase in cotton, including its characteristics and biological functions. In our study, we analyzed the sequence information, three-dimensional (3D) model, phylogenetic tree, and cis-elements of GhN/AINV23. The localization of GhN/AINV23 was determined to be in the cytoplasm and cell membrane. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that GhN/AINV23 expression was induced by abscisic acid (ABA), exogenous sucrose and low exogenous glucose, and inhibited by high exogenous glucose. In Arabidopsis, overexpression of GhN/AINV23 promoted vegetative phase change, root development, and drought tolerance. Additionally, the virus-induced gene silencing (VIGS) assay indicated that the inhibition of GhN/AINV23 expression made cotton more susceptible to drought stress, suggesting that GhN/AINV23 positively regulates plant drought tolerance. Our research indicates that GhN/AINV23 plays a significant role in plant vegetative phase change, root development, and drought response. These findings provide a valuable foundation for utilizing GhN/AINV23 to improve cotton yield.

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探索 GhN/AINV23 的作用：对植物生长、发育和耐旱性的影响

中性/碱性转化酶（N/AINVs）通过将蔗糖不可逆地水解为葡萄糖和果糖，在植物的生长、发育和胁迫反应中发挥着至关重要的作用。然而，这方面对棉花的研究还很有限。本研究旨在研究棉花中的中性/碱性转化酶 GhN/AINV23，包括其特征和生物学功能。在研究中，我们分析了 GhN/AINV23 的序列信息、三维（3D）模型、系统发生树和顺式元件。研究确定了 GhN/AINV23 在细胞质和细胞膜中的定位。实时定量聚合酶链反应（qRT-PCR）结果显示，脱落酸（ABA）、外源蔗糖和低外源葡萄糖诱导 GhN/AINV23 的表达，而高外源葡萄糖则抑制 GhN/AINV23 的表达。在拟南芥中，GhN/AINV23的过表达能促进无性期变化、根系发育和耐旱性。此外，病毒诱导基因沉默（VIGS）试验表明，抑制 GhN/AINV23 的表达会使棉花更容易受到干旱胁迫，这表明 GhN/AINV23 对植物的耐旱性有正向调节作用。我们的研究表明，GhN/AINV23 在植物无性期变化、根系发育和干旱响应中发挥着重要作用。这些发现为利用 GhN/AINV23 提高棉花产量奠定了宝贵的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biology Direct 生物-生物学

CiteScore

6.40

自引率

10.90%

发文量

审稿时长

7 months

期刊介绍： Biology Direct serves the life science research community as an open access, peer-reviewed online journal, providing authors and readers with an alternative to the traditional model of peer review. Biology Direct considers original research articles, hypotheses, comments, discovery notes and reviews in subject areas currently identified as those most conducive to the open review approach, primarily those with a significant non-experimental component.