Ligand binding properties of three odorant-binding proteins in striped flea beetle Phyllotreta striolata towards two phthalate esters

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yong Xiao, Yuhong Wu, Chunmei Lei, Fei Yin, Zhengke Peng, Xiangfeng Jing, Yongjun Zhang, Zhenyu Li
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引用次数: 0

Abstract

Odorant-binding proteins (OBPs) initiate insect olfactory perception and mediate specific binding and selection of odorants via uncertain binding mechanisms. We characterized the binding characteristics of four OBPs from the striped flea beetle Phyllotreta striolata (SFB), a major cruciferous crop pest. Tissue expression analysis revealed that the two ABPII OBPs (PstrOBP12 and PstrOBP19) were highly expressed mainly in the antenna, whereas the two minus-C OBPs (PstrOBP13 and PstrOBP16) showed a broad expression pattern. Competitive binding assays of cruciferous plant volatiles showed that PstrOBP12, PstrOBP16 and PstrOBP19 had very strong binding capacities for only two phthalate esters (Ki < 20 μM), and PstrOBP13 specifically bound to four aromatic volatiles (Ki < 11 μM). Fluorescence quenching assays displayed that two phthalate esters bound to three PstrOBPs via different quenching mechanisms. PstrOBP12/PstrOBP16–diisobutyl phthalate and PstrOBP19–bis(6-methylheptyl) phthalate followed static quenching, while PstrOBP12/PstrOBP16–bis(6-methylheptyl) phthalate and PstrOBP19–diisobutyl phthalate followed dynamic quenching. Homology modelling and molecular docking displayed that PstrOBP12–diisobutyl phthalate was driven by H-bonding and van der Waals interactions, while PstrOBP16–diisobutyl phthalate and PstrOBP19–bis(6-methylheptyl) phthalate followed hydrophobic interactions. Finally, behavioural activity analysis demonstrated that phthalate esters exhibited different behavioural activities of SFB at different doses, with low doses attracting and high doses repelling. Overall, we thus revealed the different binding properties of the three PstrOBPs to two phthalate esters, which was beneficial in shedding light on the ligand-binding mechanisms of OBPs.

Abstract Image

Abstract Image

条纹跳甲 Phyllotreta striolata 中三种气味结合蛋白对两种邻苯二甲酸酯的配体结合特性。
气味结合蛋白(OBPs)可启动昆虫的嗅觉感知,并通过不确定的结合机制介导气味的特异性结合和选择。我们研究了主要十字花科作物害虫条斑跳甲(Phyllotreta striolata,SFB)的四种 OBPs 的结合特性。组织表达分析表明,两种 ABPII OBPs(PstrOBP12 和 PstrOBP19)主要在触角中高表达,而两种 minus-C OBPs(PstrOBP13 和 PstrOBP16)则表现出广泛的表达模式。十字花科植物挥发性物质的竞争性结合试验表明,PstrOBP12、PstrOBP16 和 PstrOBP19 只对两种邻苯二甲酸酯具有很强的结合能力(Ki i
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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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