Outcomes of Simple Limbal Epithelial Transplantation Without Amniotic Membrane Grafting in Unilateral Limbal Stem Cell Deficiency: A Case Series of 6 Patients.

IF 1.9 3区 医学 Q2 OPHTHALMOLOGY
Cornea Pub Date : 2025-01-01 Epub Date: 2024-03-13 DOI:10.1097/ICO.0000000000003526
Neha Jain, Vikas Mittal, Dharma Sanandiya
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引用次数: 0

Abstract

Purpose: This study describes the technique of simple limbal epithelial transplantation (SLET) without amniotic membrane grafting (AMG) in limbal stem cell deficiency (LSCD).

Methods: Retrospective, interventional case series of 6 patients who underwent SLET without AMG were included. The procedure followed the standard technique, involving limbal biopsy from the healthy eye, resection of symblephera, and pannus dissection in the affected eye. Following host bed preparation, limbal explants were placed on the bare cornea and secured with fibrin glue. A large-diameter bandage contact lens was applied post surgery. No amniotic membrane was used. Preoperative data, including age, gender, cause of LSCD, best-corrected visual acuity, and previous ocular surgeries, were recorded. Postoperative clinical information, such as the duration of follow-up and recurrence of LSCD, best-corrected visual acuity, and other ocular examination findings, was recorded in an excel sheet.

Results: Preoperatively, 2 patients had total LSCD (secondary to a firecracker injury and excision biopsy for ocular surface squamous neoplasia). 4 patients had partial LSCD (3 chemical injuries, 1 firecracker injury). The mean age of participants was 30.67 ± 15.91 years, with a mean follow-up duration of 9.33 ± 8.04 months. Intraoperatively, all patients exhibited a smooth corneal surface after pannus removal. Postoperatively, all limbal explants remained securely attached, with complete corneal epithelialization achieved within 2 to 3 weeks. The ocular surface remained stable throughout, and no recurrence of LSCD was observed in any patient. No loss of explants was seen.

Conclusions: The present series suggests that AMG may not be a necessary step for performing SLET.

单侧睑缘干细胞缺乏症患者单纯睑缘上皮移植而非羊膜移植的疗效:6例患者的病例系列。
目的:本研究描述了在不进行羊膜移植(AMG)的情况下进行单纯瓣膜上皮移植(SLET)治疗瓣膜干细胞缺乏症(LSCD)的技术:方法:回顾性、介入性病例系列包括6名接受SLET而未接受AMG的患者。手术过程遵循标准技术,包括从健康眼球进行角膜缘活检、切除角膜巩膜和患眼的角膜脓肿。准备好宿主床后,将角膜缘外植体放在裸角膜上,并用纤维蛋白胶固定。术后戴上大直径绷带隐形眼镜。未使用羊膜。记录术前数据,包括年龄、性别、导致 LSCD 的原因、最佳矫正视力和以前的眼科手术。术后的临床信息,如随访时间和 LSCD 复发情况、最佳矫正视力和其他眼科检查结果,均记录在 excel 表中:术前,2 名患者患有完全性 LSCD(继发于爆竹伤和眼表鳞状上皮瘤切除活检),4 名患者患有部分性 LSCD(继发于爆竹伤和眼表鳞状上皮瘤切除活检)。4 名患者患有部分 LSCD(3 例化学伤,1 例鞭炮伤)。参与者的平均年龄为(30.67 ± 15.91)岁,平均随访时间为(9.33 ± 8.04)个月。术中,所有患者的角膜表面均光滑如镜。术后,所有角膜缘外植体都能牢固附着,并在 2 到 3 周内实现角膜上皮化。眼表在整个过程中保持稳定,没有发现任何患者复发 LSCD。结论:本系列研究表明,AMG可能不是进行SLET的必要步骤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cornea
Cornea 医学-眼科学
CiteScore
5.20
自引率
10.70%
发文量
354
审稿时长
3-6 weeks
期刊介绍: For corneal specialists and for all general ophthalmologists with an interest in this exciting subspecialty, Cornea brings together the latest clinical and basic research on the cornea and the anterior segment of the eye. Each volume is peer-reviewed by Cornea''s board of world-renowned experts and fully indexed in archival format. Your subscription brings you the latest developments in your field and a growing library of valuable professional references. Sponsored by The Cornea Society which was founded as the Castroviejo Cornea Society in 1975.
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