Advancing Diagnostics: Evaluating the Potential of Nanoparticle-Based Immunoassays as PCR Alternatives for Disease Confirmation

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-12 DOI:10.2174/0115734110287852240228064645

Aditya Kurdekar, Venkataramaniah Kamisetti

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Abstract

: In diagnostic technologies, searching for quick, accurate, and cost-efficient ways to confirm diseases has prompted the investigation of alternative methodologies to classic PCR-based tests. This article delves into the growing field of nanoparticle-based immunoassays, offering a comprehensive evaluation of their potential as viable alternatives to PCR for disease diagnostics. We discuss the basic principles of nanoparticle-based immunoassays, highlighting their distinctive capacity to combine the specialized binding characteristics of antibodies with the improved detection capacities of nanoparticles. The article compares the performance attributes of nanoparticle-based immunoassays compared to PCR, focusing on sensitivity, specificity, and detection speed. We further delve into a novel diagnostic technology, immuno-PCR, which integrates the strengths of immunoassays and PCR techniques. Healthcare systems and stakeholders must comprehend and implement novel diagnostic procedures emphasizing accuracy and sensitivity as the diagnostic landscape advances globally. This review contributes to the advancement of diagnostics by synthesizing current research and suggesting future directions for development. It highlights the transformative potential of current and emerging methods in shaping the future of disease diagnostics.

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推进诊断：评估基于纳米粒子的免疫测定作为疾病确认的 PCR 替代品的潜力

:在诊断技术中，寻找快速、准确、经济高效的方法来确诊疾病促使人们研究传统 PCR 测试的替代方法。本文深入探讨了不断发展的基于纳米粒子的免疫测定领域，对其作为疾病诊断中 PCR 的可行替代方法的潜力进行了全面评估。我们讨论了基于纳米粒子的免疫测定的基本原理，强调了它们将抗体的专门结合特性与纳米粒子的改进检测能力相结合的独特能力。文章比较了基于纳米粒子的免疫测定与 PCR 的性能属性，重点关注灵敏度、特异性和检测速度。我们还进一步深入探讨了一种新型诊断技术--免疫 PCR，它整合了免疫测定和 PCR 技术的优势。随着全球诊断技术的发展，医疗保健系统和利益相关者必须理解并实施强调准确性和灵敏度的新型诊断程序。本综述综合了当前的研究成果，并提出了未来的发展方向，有助于推动诊断技术的发展。它强调了当前和新兴方法在塑造疾病诊断未来方面的变革潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.