Molecular differentiation of the green and purple Tulsi (Ocimum tenuiflorum L.) and its application in authentication of market samples

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-11 DOI:10.1007/s13562-024-00883-3

Mohamed Shaibi, Raju Balaji, Madasamy Parani

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Abstract

Tulsi (Holi Basil, Ocimum tenuiflorum) is extensively used in herbal medicine, and it includes two distinct subtypes; namely green Tulsi and purple Tulsi. Both types have similar medicinal properties. However, purple Tulsi contains a significantly higher amount of methyleugenol, which is genotoxic, and its daily intake is restricted. We developed a polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) method to identify purple Tulsi. For this purpose, we selected a C > T single nucleotide polymorphism (SNP) in the ycf1 gene of the chloroplast genome that overlapped with a DdeI restriction site. The primers and PCR conditions were optimized to amplify a 797 bp DNA encompassing the C > T SNP, specifically from O. tenuiflorum. After restriction digestion of the PCR product with DdeI, green Tulsi was identified by two fragments (539 bp and 258 bp), and purple Tulsi was recognized by a single fragment (797 bp). Analysis of 40 Tulsi market samples revealed that only 36 (90%) were derived from O. tenuiflorum. The majority of the market samples were purple Tulsi (60%) or a mixture of green and purple Tulsi (27.5%), with some mixed samples containing up to 50% purple Tulsi.

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绿色和紫色 Tulsi（Ocimum tenuiflorum L.）的分子分化及其在市场样本鉴定中的应用

图尔西（霍利罗勒，Ocimum tenuiflorum）被广泛用于草药，它包括两种不同的亚型，即绿色图尔西和紫色图尔西。这两种植物的药效相似。然而，紫图尔西含有较高的甲基丁香酚，具有遗传毒性，因此限制了其每日摄入量。我们开发了一种聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）方法来鉴定紫色图尔西。为此，我们选择了叶绿体基因组 ycf1 基因中与 DdeI 限制位点重叠的 C > T 单核苷酸多态性（SNP）。对引物和 PCR 条件进行了优化，以扩增包含 C > T SNP 的 797 bp DNA，特别是来自 O. tenuiflorum 的 DNA。用 DdeI 对 PCR 产物进行限制性消化后，通过两个片段（539 bp 和 258 bp）识别出绿色图尔西，通过一个片段（797 bp）识别出紫色图尔西。对 40 个图尔西市场样品的分析表明，只有 36 个（90%）来自 O. tenuiflorum。大多数市场样品是紫色图尔西（60%）或绿色和紫色图尔西的混合物（27.5%），一些混合样品中紫色图尔西的含量高达 50%。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.