Genetic and phenotypic diversity in Solanum lycopersicum genotypes: insights from morpho-molecular and biochemical analyses

IF 1.7 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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Abstract

Tomato (Solanum lycopersicum L.) crop is well-known for its versatility worldwide and is also recognized as model species used extensively for various genetic studies. The aim of this research was to investigate both inter and intra-genetic diversity present among various tomato genotypes. This investigation was carried out through a comprehensive analysis encompassing morphological observations, biochemical assessments, and the utilization of SSR markers. A total of 15 discrete agro-morphological traits and six biochemical traits were undertaken in the current study for evaluating the analysis of variance, genetic parameters and correlation. The analysis of variance indicated significant differences across genotypes for all 15 agro-morphological traits and 6 biochemical traits tested, indicating that the experimental material included considerable variability. Morphological clustering divided the genotypes into 2 clusters and the genotype wise distance matrix was obtained to identify the most diverse genotypes. PCA analysis was conducted to understand the directive relation of traits and magnitude of variability contributed by them. SSR profiling with 24 primers identified 44 alleles with 1.83 as mean number of alleles/SSR with an average PIC value of 0.31. Structure analysis revealed two sub-populations (K = 2). The AMOVA indicated that 98% of the total variation was present within populations. This study presents a roadmap for composing future breeding strategies for integrating desirable traits in novel tomato lines that combine robustness and nutritive value.

番茄红素基因型的遗传和表型多样性:形态-分子和生化分析的启示
摘要 番茄(Solanum lycopersicum L.)因其用途广泛而闻名于世,也被公认为广泛用于各种遗传研究的模式物种。本研究的目的是调查各种番茄基因型之间和基因内部的多样性。这项研究是通过形态观察、生化评估和 SSR 标记利用等综合分析进行的。本研究共对 15 个离散农业形态特征和 6 个生化特征进行了方差分析、遗传参数和相关性评估。方差分析结果表明,在所有 15 个农业形态特征和 6 个生化特征测试中,不同基因型之间存在显著差异,这表明实验材料具有相当大的变异性。形态聚类将基因型分为 2 个聚类,并获得了基因型间的距离矩阵,以确定差异最大的基因型。通过 PCA 分析,了解了性状之间的指导关系及其变异程度。使用 24 个引物进行的 SSR 分析确定了 44 个等位基因,平均等位基因数/SSR 为 1.83,平均 PIC 值为 0.31。结构分析显示有两个亚群(K = 2)。AMOVA表明,98%的总变异存在于种群内部。这项研究为今后制定育种策略提供了路线图,以便在兼具稳健性和营养价值的新型番茄品系中整合理想的性状。
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来源期刊
Plant Biotechnology Reports
Plant Biotechnology Reports 生物-生物工程与应用微生物
CiteScore
4.10
自引率
4.20%
发文量
72
审稿时长
>12 weeks
期刊介绍: Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.
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