Extrusion 3D-printed tricalcium phosphate-polycaprolactone biocomposites for quercetin-KCl delivery in bone tissue engineering

IF 3.9 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Connor Toulou, Vishal Sharad Chaudhari, Susmita Bose
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Abstract

Critical-sized bone defects pose a significant challenge in advanced healthcare due to limited bone tissue regenerative capacity. The complex interplay of numerous overlapping variables hinders the development of multifunctional biocomposites. Phytochemicals show promise in promoting bone growth, but their dose-dependent nature and physicochemical properties halt clinical use. To develop a comprehensive solution, a 3D-printed (3DP) extrusion-based tricalcium phosphate-polycaprolactone (TCP-PCL) scaffold is augmented with quercetin and potassium chloride (KCl). This composite material demonstrates a compressive strength of 30 MPa showing promising stability for low load-bearing applications. Quercetin release from the scaffold follows a biphasic pattern that persists for up to 28 days, driven via diffusion-mediated kinetics. The incorporation of KCl allows for tunable degradation rates of scaffolds and prevents the initial rapid release. Functionalization of scaffolds facilitates the attachment and proliferation of human fetal osteoblasts (hfOB), resulting in a 2.1-fold increase in cell viability. Treated scaffolds exhibit a 3-fold reduction in osteosarcoma (MG-63) cell viability as compared to untreated substrates. Ruptured cell morphology and decreased mitochondrial membrane potential indicate the antitumorigenic potential. Scaffolds loaded with quercetin and quercetin-KCl (Q-KCl) demonstrate 76% and 89% reduction in bacterial colonies of Staphylococcus aureus, respectively. This study provides valuable insights as a promising strategy for bone tissue engineering (BTE) in orthopedic repair.

Abstract Image

Abstract Image

用于骨组织工程中槲皮素-氯化钾递送的挤压三维打印磷酸三钙-聚己内酯生物复合材料。
由于骨组织再生能力有限,临界大小的骨缺损给先进的医疗保健带来了巨大挑战。众多重叠变量的复杂相互作用阻碍了多功能生物复合材料的开发。植物化学物质有望促进骨骼生长,但其剂量依赖性和理化特性阻碍了其临床应用。为了开发一种全面的解决方案,一种基于三维打印(3DP)挤压技术的磷酸三钙-聚己内酯(TCP-PCL)支架添加了槲皮素和氯化钾(KCl)。这种复合材料的抗压强度为 30 兆帕,在低承重应用中显示出良好的稳定性。槲皮素从支架中的释放遵循双相模式,通过扩散介导的动力学作用可持续长达 28 天。氯化钾的加入可以调节支架的降解速率,并防止最初的快速释放。支架的功能化可促进人类胎儿成骨细胞(hfOB)的附着和增殖,使细胞存活率提高 2.1 倍。与未经处理的基底相比,经过处理的支架使骨肉瘤(MG-63)细胞的存活率降低了 3 倍。破裂的细胞形态和线粒体膜电位的降低表明了抗肿瘤潜力。负载了槲皮素和槲皮素-氯化钾(Q-KCl)的支架分别减少了 76% 和 89% 的金黄色葡萄球菌细菌菌落。这项研究为骨组织工程(BTE)在骨科修复中的应用提供了有价值的见解。
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来源期刊
Journal of biomedical materials research. Part A
Journal of biomedical materials research. Part A 工程技术-材料科学:生物材料
CiteScore
10.40
自引率
2.00%
发文量
135
审稿时长
3.6 months
期刊介绍: The Journal of Biomedical Materials Research Part A is an international, interdisciplinary, English-language publication of original contributions concerning studies of the preparation, performance, and evaluation of biomaterials; the chemical, physical, toxicological, and mechanical behavior of materials in physiological environments; and the response of blood and tissues to biomaterials. The Journal publishes peer-reviewed articles on all relevant biomaterial topics including the science and technology of alloys,polymers, ceramics, and reprocessed animal and human tissues in surgery,dentistry, artificial organs, and other medical devices. The Journal also publishes articles in interdisciplinary areas such as tissue engineering and controlled release technology where biomaterials play a significant role in the performance of the medical device. The Journal of Biomedical Materials Research is the official journal of the Society for Biomaterials (USA), the Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Articles are welcomed from all scientists. Membership in the Society for Biomaterials is not a prerequisite for submission.
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