A genome-wide association study for resistance to Fusarium wilt (Fusarium oxysporum f. sp. vasinfectum) race 4 in diploid cotton (Gossypium arboreum) and resistance transfer to tetraploid Gossypium hirsutum.

IF 2.3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Abdelraheem Abdelraheem, Yi Zhu, Linghe Zeng, Salliana Stetina, Jinfa Zhang
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Abstract

Fusarium wilt, caused by the soilborne fungus Fusarium oxysporum f. sp. vasinfectum (FOV), is a devastating disease affecting cotton (Gossypium spp.) worldwide. Understanding the genetic basis of resistance in diploid cotton and successfully transferring the resistance to tetraploid Upland cotton (G. hirsutum) are crucial for developing resistant cotton cultivars. Although numerous studies have been conducted to investigate the genetic basis of Fusarium wilt in tetraploid cotton, little research has been conducted on diploid species. In this study, an association mapping panel consisting of 246 accessions of G. arboreum, was used to identify chromosomal regions for FOV race 4 (FOV4) resistance based on foliar disease severity ratings in four greenhouse tests. Through a genome-wide association study (GWAS) based on 7,009 single nucleotide polymorphic (SNP) markers, 24 FOV4 resistance QTLs, including three major QTLs on chromosomes A04, A06, and A11, were detected. A validation panel consisting of 97 diploid cotton accessions was employed, confirming the presence of several QTLs. Evaluation of an introgressed BC2F7 population derived from G. hirsutum/G. aridum/G. arboreum showed significant differences in disease incidence and mortality rate, as compared to susceptible and resistant controls, suggesting that the resistance in G. arboreum and/or G. aridum was transferred into Upland cotton for the first time. The identification of novel major resistance QTLs, along with the transfer of resistance from the diploid species, expands our understanding of the genomic regions involved in conferring resistance to FOV4 and contributes to the development of resilient Upland cotton cultivars.

Abstract Image

二倍体棉花(Gossypium arboreum)抗镰刀菌枯萎病(Fusarium oxysporum f. sp. vasinfectum)第 4 种族的全基因组关联研究以及向四倍体棉花(Gossypium hirsutum)的抗性转移。
由土壤传播真菌 Fusarium oxysporum f. sp. vasinfectum(FOV)引起的镰刀菌枯萎病是影响全球棉花(Gossypium spp.)的毁灭性病害。了解二倍体棉花抗性的遗传基础,并成功地将抗性转移到四倍体陆地棉(G. hirsutum)上,对于开发抗性棉花栽培品种至关重要。虽然已有大量研究调查了四倍体棉花镰刀菌枯萎病的遗传基础,但针对二倍体棉花的研究却很少。本研究使用了一个由 246 个 G. arboreum 品种组成的关联图谱小组,根据四个温室测试中的叶片病害严重程度评级,确定了 FOV race 4(FOV4)抗性的染色体区域。通过基于 7,009 个单核苷酸多态性(SNP)标记的全基因组关联研究(GWAS),发现了 24 个 FOV4 抗性 QTLs,包括染色体 A04、A06 和 A11 上的三个主要 QTLs。由 97 个二倍体棉花品种组成的验证小组证实了多个 QTLs 的存在。对源自 G. hirsutum/G. aridum/G. arboreum 的引种 BC2F7 群体的评估显示,与易感对照和抗性对照相比,该群体的发病率和死亡率存在显著差异,这表明 G. arboreum 和/或 G. aridum 的抗性首次转移到了陆地棉中。新的主要抗性 QTLs 的鉴定以及二倍体物种抗性的转移,扩大了我们对赋予 FOV4 抗性的基因组区域的了解,有助于开发抗逆性强的陆地棉栽培品种。
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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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