Dissecting the dynamic cellular transcriptional atlas of adult teleost testis development throughout the annual reproductive cycle.

IF 3.7 2区 生物学 Q1 DEVELOPMENTAL BIOLOGY
Development Pub Date : 2024-10-15 Epub Date: 2024-03-13 DOI:10.1242/dev.202296
Chaofan Jin, Kai Yan, Mengya Wang, Weihao Song, Bo Wang, Yu Men, Jingjing Niu, Yan He, Quanqi Zhang, Jie Qi
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引用次数: 0

Abstract

Teleost testis development during the annual cycle involves dramatic changes in cellular compositions and molecular events. In this study, the testicular cells derived from adult black rockfish at distinct stages - regressed, regenerating and differentiating - were meticulously dissected via single-cell transcriptome sequencing. A continuous developmental trajectory of spermatogenic cells, from spermatogonia to spermatids, was delineated, elucidating the molecular events involved in spermatogenesis. Subsequently, the dynamic regulation of gene expression associated with spermatogonia proliferation and differentiation was observed across spermatogonia subgroups and developmental stages. A bioenergetic transition from glycolysis to mitochondrial respiration of spermatogonia during the annual developmental cycle was demonstrated, and a deeper level of heterogeneity and molecular characteristics was revealed by re-clustering analysis. Additionally, the developmental trajectory of Sertoli cells was delineated, alongside the divergence of Leydig cells and macrophages. Moreover, the interaction network between testicular micro-environment somatic cells and spermatogenic cells was established. Overall, our study provides detailed information on both germ and somatic cells within teleost testes during the annual reproductive cycle, which lays the foundation for spermatogenesis regulation and germplasm preservation of endangered species.

剖析成鱼睾丸在整个年度生殖周期中的动态细胞转录图谱。
远洋鱼类睾丸在年周期中的发育涉及细胞组成和分子事件的巨大变化。在这项研究中,我们通过单细胞转录组测序技术,对来自成年黑岩鱼的睾丸细胞在不同阶段--退行期、再生期和分化期--进行了细致的解剖。研究人员描绘了生精细胞从精原细胞到精母细胞的连续发育轨迹,阐明了精子发生过程中的分子事件。随后,在精原细胞亚群和各发育阶段观察到了与精原细胞增殖和分化相关的基因表达动态调控。研究证明了精原细胞在年发育周期中从糖酵解到线粒体呼吸的生物能转变,并通过再聚类分析揭示了更深层次的异质性和分子特征。此外,研究人员还勾勒出 Sertoli 细胞的发育轨迹,以及 Leydig 细胞和巨噬细胞的分化过程。此外,还建立了睾丸微环境体细胞与生精细胞之间的相互作用网络。总之,我们的研究提供了每年生殖周期中远缘动物睾丸内生殖细胞和体细胞的详细信息,为濒危物种的精子发生调控和种质保存奠定了基础。
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来源期刊
Development
Development 生物-发育生物学
CiteScore
6.70
自引率
4.30%
发文量
433
审稿时长
3 months
期刊介绍: Development’s scope covers all aspects of plant and animal development, including stem cell biology and regeneration. The single most important criterion for acceptance in Development is scientific excellence. Research papers (articles and reports) should therefore pose and test a significant hypothesis or address a significant question, and should provide novel perspectives that advance our understanding of development. We also encourage submission of papers that use computational methods or mathematical models to obtain significant new insights into developmental biology topics. Manuscripts that are descriptive in nature will be considered only when they lay important groundwork for a field and/or provide novel resources for understanding developmental processes of broad interest to the community. Development includes a Techniques and Resources section for the publication of new methods, datasets, and other types of resources. Papers describing new techniques should include a proof-of-principle demonstration that the technique is valuable to the developmental biology community; they need not include in-depth follow-up analysis. The technique must be described in sufficient detail to be easily replicated by other investigators. Development will also consider protocol-type papers of exceptional interest to the community. We welcome submission of Resource papers, for example those reporting new databases, systems-level datasets, or genetic resources of major value to the developmental biology community. For all papers, the data or resource described must be made available to the community with minimal restrictions upon publication. To aid navigability, Development has dedicated sections of the journal to stem cells & regeneration and to human development. The criteria for acceptance into these sections is identical to those outlined above. Authors and editors are encouraged to nominate appropriate manuscripts for inclusion in one of these sections.
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