PRPF19 mRNA Encodes a Small Open Reading Frame That Is Important for Viability of Human Cells

IF 0.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Doklady Biochemistry and Biophysics Pub Date : 2024-03-12 DOI:10.1134/S1607672923700722

N. M. Shepelev, A. O. Kurochkina, O. A. Dontsova, M. P. Rubtsova

引用次数: 0

Abstract

High-throughput ribosome profiling demonstrates the translation of thousands of small open reading frames located in the 5′ untranslated regions of messenger RNAs (upstream ORFs). Upstream ORF can both perform a regulatory function by influencing the translation of the downstream main ORF and encode a small functional protein or microprotein. In this work, we showed that the 5′ untranslated region of the PRPF19 mRNA encodes an upstream ORF that is translated in human cells. Inactivation of this upstream ORF reduces the viability of human cells.

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PRPF19 mRNA 编码一个小型开放阅读框，对人类细胞的存活率非常重要。

高通量核糖体分析显示，位于信使 RNA（上游 ORF）5'非翻译区的数千个小型开放阅读框在进行翻译。上游 ORF 既能通过影响下游主 ORF 的翻译发挥调控功能，也能编码小型功能蛋白或微量蛋白。在这项工作中，我们发现 PRPF19 mRNA 的 5' 非翻译区编码一个上游 ORF，该 ORF 在人体细胞中被翻译。该上游 ORF 失活会降低人体细胞的活力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Doklady Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.