Distance-based paper analytical device for multiplexed quantification of cytokine biomarkers using carbon dots integrated with molecularly imprinted polymer†

IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2024-03-12 DOI:10.1039/D4LC00055B
Kawin Khachornsakkul, Ruben Del-Rio-Ruiz, Lita Chheang, Wenxin Zeng and Sameer Sonkusale
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Abstract

This article introduces distance-based paper analytical devices (dPADs) integrated with molecularly imprinted polymers (MIPs) and carbon dots (CDs) for simultaneous quantification of cytokine biomarkers, namely C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) in human biological samples for diagnosis of cytokine syndrome. Using fluorescent CDs and MIP technology, the dPAD exhibits high selectivity and sensitivity. Detection is based on fluorescence quenching of CDs achieved through the interaction of the target analytes with the MIP layer on the paper substrate. Quantitative analysis is easily accomplished by measuring the distance length of quenched fluorescence with a traditional ruler and naked eye readout enabling rapid diagnosis of cytokine syndrome and the underlying infection. Our sensor demonstrated linear ranges of 2.50–24.0 pg mL−1 (R2 = 0.9974), 0.25–3.20 pg mL−1 (R2 = 0.9985), and 1.50–16.0 pg mL−1 (R2 = 0.9966) with detection limits (LODs) of 2.50, 0.25, and 1.50 pg mL−1 for CRP, TNF-α, and IL-6, respectively. This sensor also demonstrated remarkable selectivity compared to a sensor employing a non-imprinted polymer (NIP), and precision with the highest relative standard deviation (RSD) of 5.14%. The sensor is more accessible compared to prior methods relying on expensive reagents and instruments and complex fabrication methods. Furthermore, the assay provided notable accuracy for monitoring these biomarkers in various human samples with recovery percentages ranging between 99.22% and 103.58%. By integrating microfluidic systems, nanosensing, and MIPs technology, our developed dPADs hold significant potential as a cost-effective and user-friendly analytical method for point-of-care diagnostics (POC) of cytokine-related disorders. This concept can be further extended to developing diagnostic devices for other biomarkers.

Abstract Image

利用碳点与分子印迹聚合物集成的基于距离的纸质分析装置,对细胞因子生物标记物进行多重定量分析
本文介绍了集成了分子印迹聚合物(MIPs)和碳点(CDs)的距离纸分析装置(dPADs),用于同时定量检测人体生物样本中的细胞因子生物标志物,即 C 反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6),以诊断细胞因子综合征。dPAD 采用荧光 CD 和 MIP 技术,具有高选择性和高灵敏度。检测是通过目标分析物与纸基底上的 MIP 层相互作用实现 CD 的荧光淬灭。利用传统的尺子和肉眼读数测量淬灭荧光的距离长度,就能轻松实现定量分析,从而快速诊断细胞因子综合征和潜在感染。我们的传感器对 CRP、TNF-α 和 IL-6 的线性范围分别为 2.50-24.0 pg mL-1(R2 = 0.9974)、0.25-3.20 pg mL-1(R2 = 0.9985)和 1.50-16.0 pg mL-1(R2 = 0.9966),检测限分别为 2.50、0.25 和 1.50 pg mL-1。与使用非压印聚合物(NIP)的传感器相比,该传感器还具有显著的选择性和精确性,相对标准偏差(RSD)最高为 5.14%。与之前依赖昂贵试剂、仪器和复杂制造方法的方法相比,该传感器更易于使用。此外,该检测方法在监测各种人体样本中的这些生物标记物时具有明显的准确性,回收率在 99.22% 到 103.58% 之间。通过整合微流控系统、纳米传感和 MIPs 技术,我们开发的 dPADs 具有巨大的潜力,可作为细胞因子相关疾病的床旁诊断 (POC) 的一种成本效益高且用户友好的分析方法。这一概念还可进一步扩展到其他生物标记物的诊断设备开发。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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