Effects of Mesenchymal Stem Cell-conditioned Media with Natural Immunomodulatory Agent Resveratrol on Type 1 Diabetes.

Krushna Abhale, Addepalli Veeranjaneyulu, Shivani Desai, Avinash Sanap, Ramesh Bhonde
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Abstract

Background: Type 1 diabetes mellitus (T1DM) is a condition marked by elevated blood sugar levels and primarily recognized by the destruction of beta cells caused by an autoimmune attack, which is a significant characteristic of T1DM. Recent studies have demonstrated the regenerative potential of conditioned medium therapy. In light of this, the current research sought to assess the impact of Mesenchymal Stem Cell conditioned media (CM) and CM with resveratrol (CM+ Resveratrol) on the management of T1DM in Swiss albino mice. By leveraging and modifying existing conditioned medium therapy, this study aims to evaluate its effectiveness in treating T1DM.

Materials & methods: Diabetes was induced in animals using the diabetes-inducing agent streptozotocin (STZ). The animals were then divided into five groups: Normal control, Disease Control, Resveratrol, Condition Media, and CM + Resveratrol. Treatments were given to the animals accordingly. The study period was 28 days. During this time, the animals were monitored for foodwater intake twice a week, blood glucose levels, and body weight. At the conclusion of the 28-day study period, biochemical estimations were performed for serum insulin levels, C-peptide levels, anti-inflammatory cytokines levels and pro-inflammatory cytokines levels. Additionally, histopathology of the pancreas was performed.

Results: The test groups showed a significant decrease in blood glucose levels, an increase in Cpeptide levels, and a decrease in pro-inflammatory cytokine levels compared to the disease group. However, no statistically significant change within groups was observed in terms of serum insulin and anti-inflammatory cytokine levels. The improvement in diabetic symptoms, such as polyphagia, polydipsia, and weight loss, was observed in the treatment group, along with pancreatic regeneration, which indicated improved insulin secretion.

Conclusion: In the current investigation, we concluded that CM and CM+ Resveratrol, as natural immunomodulators, have the capacity to regenerate injured pancreatic beta cells and have antidiabetic action, together with immunomodulating impact. Nonetheless, future studies on this therapy appear to be promising.

间充质干细胞条件培养基与天然免疫调节剂白藜芦醇对 1 型糖尿病的影响
背景:1 型糖尿病(T1DM)是一种以血糖水平升高为特征的疾病,主要由自身免疫攻击导致的β细胞破坏引起,这是 T1DM 的一个显著特征。最近的研究表明,条件培养基疗法具有再生潜力。有鉴于此,目前的研究试图评估间充质干细胞条件培养基(CM)和含有白藜芦醇的条件培养基(CM+白藜芦醇)对瑞士白化小鼠 T1DM 治疗的影响。通过利用和修改现有的条件培养基疗法,本研究旨在评估其治疗 T1DM 的有效性:使用糖尿病诱导剂链脲佐菌素(STZ)诱导动物患糖尿病。然后将动物分为五组:正常对照组、疾病对照组、白藜芦醇组、条件培养基组和 CM + 白藜芦醇组。对动物进行相应的治疗。研究期为 28 天。在此期间,每周两次监测动物的食水摄入量、血糖水平和体重。28 天研究结束后,对血清胰岛素水平、C 肽水平、抗炎细胞因子水平和促炎细胞因子水平进行生化评估。此外,还对胰腺进行了组织病理学检查:结果:与疾病组相比,试验组的血糖水平明显下降,肌肽水平上升,促炎细胞因子水平下降。然而,在血清胰岛素和抗炎细胞因子水平方面,组内没有观察到明显的统计学变化。治疗组的多食、多饮和体重减轻等糖尿病症状有所改善,胰腺再生也有所改善,这表明胰岛素分泌有所改善:在目前的研究中,我们得出结论,中药和中药+白藜芦醇作为天然的免疫调节剂,有能力再生受伤的胰腺β细胞,并具有抗糖尿病作用和免疫调节作用。尽管如此,未来对这种疗法的研究仍大有可为。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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